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Gene Expression Data
Assay Details
Assay
Reference: J:279323 Hagan AS, et al., Generation and validation of novel conditional flox and inducible Cre alleles targeting fibroblast growth factor 18 (Fgf18). Dev Dyn. 2019 Sep;248(9):882-893
Assay type: Recombinase reporter
MGI Accession ID: MGI:6385557
Gene symbol: Fgf18
Gene name: fibroblast growth factor 18
Reporter: tdTomato
Direct Detection
Results
Specimen 4G: embryonic day 8.5; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen (80 mg/kg) and progesterone (40 mg/kg) were co-injected intraperitoneally (IP) into pregnant dams 24 hours before collection of embryos.
Structure Level Pattern Image Note
TS13: somite Present 4G
TS13: allantois Present Activiy found in a few cells in the mid-hindbrain junction (mhb) and allontois.
TS13: midbrain-hindbrain junction Present Activiy found in a few cells in the mid-hindbrain junction (mhb) and allontois.
Specimen 4H: embryonic day 10.5; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen (80 mg/kg) and progesterone (40 mg/kg) were co-injected intraperitoneally (IP) into pregnant dams 24 hours before collection of embryos.
Structure Level Pattern Image Note
TS17: midbrain-hindbrain junction Present 4H
TS17: somite Present 4H
Specimen 4I: embryonic day 13.5; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen (80 mg/kg) and progesterone (40 mg/kg) were co-injected intraperitoneally (IP) into pregnant dams 24 hours before collection of embryos.
Structure Level Pattern Image Note
TS21: forelimb Present 4I
TS21: forelimb digit Present 4I
TS21: hindlimb Present 4I
TS21: hindlimb digit Present 4I
TS21: jaw Present 4I
TS21: somite Present 4I
TS21: back of trunk Present
TS21: limb interdigital region mesenchyme Present
Specimen 4 not shown: embryonic day 10.5; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen (80 mg/kg) and progesterone (40 mg/kg) were co-injected intraperitoneally (IP) into pregnant dams 24 hours before collection of embryos.
Structure Level Pattern Image Note
TS17: forebrain Present Single cells low level activity in the forebrain as well as individual cells in a salt-and pepper pattern in the tailbud and future jaw.
TS17: oral region Present Single cells low level activity in the forebrain as well as individual cells in a salt-and pepper pattern in the tailbud and future jaw.
TS17: tail Present Single cells low level activity in the forebrain as well as individual cells in a salt-and pepper pattern in the tailbud and future jaw.
Specimen 5A: postnatal day 9; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by IP injection at a dose of 150 g on four sequential days beginning at postnatal day 5.
Structure Level Pattern Image Note
TS28: joint articular surface Present 5A'' , 5A , 5A'
TS28: meniscus of knee Present 5A'' , 5A , 5A'
TS28: periosteum Present 5A'' , 5A , 5A'
Specimen 5B: postnatal day 21; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by IP injection at a dose of 150 g on four sequential days beginning at postnatal day 5.
Structure Level Pattern Image Note
TS28: periosteum Present 5B' , 5B Also present in the groove of Ranvier.
Specimen 5C: postnatal day 56; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by IP injection at a dose of 150 g on four sequential days beginning at postnatal day 5.
Structure Level Pattern Image Note
TS28: joint articular surface Present 5C' , 5C
TS28: periosteum Present 5C' , 5C
Specimen 5D: postnatal week 12; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by chow (400 mg/kg tamoxifen citrate) at 8 weeks for 14 days.
Structure Level Pattern Image Note
TS28: joint articular surface Present 5D' , 5D
TS28: periosteum Present 5D' , 5D
Specimen 6A-D: postnatal day 9; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by IP injection at a dose of 150 g on four sequential days beginning at postnatal day 5.
Structure Level Pattern Image Note
TS28: bladder Absent
TS28: metanephros Absent
TS28: small intestine Absent
TS28: spleen Present 6A spleen seen in the sub-mesothelial connective tissue capsule as well as sparse cells in the parenchyma
TS28: lung Present Single cells 6B lung present in mesothelium cells and many cells in the parenchyma predominantly alveolar myofibroblasts
TS28: liver Present 6C liver in perivascular regions
TS28: pancreas Present 6D pancreas in perivascular regions
Specimen 7A-G: postnatal week 10; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by chow (400 mg/kg tamoxifen citrate) at 8 weeks for 7 days followed by a 1-week washout before tissue collection.
Structure Level Pattern Image Note
TS28: pancreas Absent
TS28: testis Absent
TS28: white fat Absent
TS28: spleen Present 7A spleen , 7A' spleen in capsule and in the perivascular region around large vessels
TS28: aorta Present 7C aorta in the outermost mesenchymal derived interstitial cells (adventitia) of the vascular wall
TS28: heart Present 7B heart , 7G heart valve in visceral mesothelial cells and also abundant in cardiac valve interstitial cells
TS28: heart valve Present 7B heart , 7G heart valve in visceral mesothelial cells and also abundant in cardiac valve interstitial cells
TS28: lung Strong 7D lung in visceral mesothelial cells
TS28: liver Strong 7E liver in visceral mesothelial cells
TS28: metanephros Present 7F kidney in visceral mesothelial cells
Specimen 8B,E-O: postnatal week 12; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by chow (400 mg/kg tamoxifen citrate) at 9 weeks for 2 weeks followed by a 1-week washout before tissue collection.
Structure Level Pattern Image Note
TS28: hypothalamus Present 8F
TS28: cerebral cortex Present 8B , 8G in a subset of cortical pial astrocytes
TS28: 3rd ventricle choroid plexus Present 8E , 8F
TS28: 3rd ventricle ependyma Present 8E , 8F
TS28: telencephalon septum Present 8J
TS28: lateral ventricle Present 8M , 8O , 8J , 8N , 8L' in the walls of the lateral ventricles
TS28: main olfactory bulb Present 8M , 8O , 8J , 8N , 8L' in the walls of the lateral ventricles
TS28: central amygdaloid nucleus Present
TS28: 4th ventricle choroid plexus Present 8H
Specimen 8C-D: postnatal week 12; Fgf18tm2.2(cre/ERT2)Dor/Fgf18+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen was administered by chow (400 mg/kg tamoxifen citrate) at 9 weeks for 2 weeks followed by a 1-week washout before tissue collection.
Structure Level Pattern Image Note
TS28: cerebral cortex Present Single cells 8D , 8C in rare neurons in the cerebral cortex
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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