Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:279805
Baleriola J, et al., Increased neuronal death and disturbed axonal growth in the Polmu-deficient mouse embryonic retina. Sci Rep. 2016 May 13;6:25928
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6383841
|
|
Gene symbol:
|
Tubb3
|
|
Gene name:
|
tubulin, beta 3 class III
|
|
Antibody:
|
Anti-Tubb3
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
|
Results
|
Specimen
3d:
embryonic day 13.5
(more  )
Specimen
3d:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5b:
(close )
|
Genetic Background:
|
B6.129-Polmtm1Abnd/Abnd
|
|
Mutant Allele(s):
|
Polmtm1Abnd/Polmtm1Abnd
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
5b
|
Expression was detected in retinal ganglion cell axons in the dorsal retina field. In this mutant sample, axonal fasciculation was severely defective.
|
Specimen
5c:
embryonic day 13.5
(more )
Specimen
5c:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
5c
|
Expression was detected in retinal ganglion cell axons in the dorsal retina field.
|
Specimen
5d:
(close )
|
Genetic Background:
|
B6.129-Polmtm1Abnd/Abnd
|
|
Mutant Allele(s):
|
Polmtm1Abnd/Polmtm1Abnd
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
5d
|
Expression was detected in retinal ganglion cell axons in the dorsal retina field. In this mutant sample, axonal fasciculation was mildly defective.
|
Specimen
5e:
embryonic day 13.5
(more )
Specimen
5e:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
5e
|
Expression was detected in retinal ganglion cell axons in the dorsal retina field.
|
Specimen
5f:
(close )
|
Genetic Background:
|
B6.129-Polmtm1Abnd/Abnd
|
|
Mutant Allele(s):
|
Polmtm1Abnd/Polmtm1Abnd
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
5f
|
Expression was detected in retinal ganglion cell axons in the dorsal retina field. In this mutant sample, axonal fasciculation was normal.
|
Specimen
8a:
embryonic day 13.5
(more )
Specimen
8a:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
8a
|
Expression was observed in both the soma and the axons of retinal ganglion cells. Staining delimited an area adjacent to the vitreal surface of the retina containing most of the retinal ganglion cell bodies.
|
Specimen
8b:
(close )
|
Genetic Background:
|
B6.129-Polmtm1Abnd/Abnd
|
|
Mutant Allele(s):
|
Polmtm1Abnd/Polmtm1Abnd
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
8b
|
Staining in the mutant was reduced compared to wild type. In addition, a higher number of ectopic immunopositive cells were outside the continuously stained area.
|
|
Analysis Tools