Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135151
Sigulinsky CL, et al., Vsx2/Chx10 ensures the correct timing and magnitude of Hedgehog signaling in the mouse retina. Dev Biol. 2008 May 15;317(2):560-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6383012
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 probe37
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The genetic background of the wild type specimens was either +/+ or heterozygous orJ. The authors report these mice to be phenotypically indistinguishable and considered to be equivalent.
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
1A
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Expression in retinal progenitor cells (RPCs), restricted to central retina and stronger on the dorsal side
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| TS20: perioptic mesenchyme |
Strong |
Regionally restricted |
1A
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Strong expression in extra-retinal tissue.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Widespread |
2C
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Expression in retinal progenitor cells (RPCs) extends to peripheral retina.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
2D
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Expression is centrally restricted. Where expressed, level of expression appear normal compared to wild type.
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Specimen
3B/C:
embryonic day 15.5
(more )
Note: Double labeled: blue - Gli1; green - Tubb3 (assay MGI:6383021).
Specimen
3B/C:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gli1; green - Tubb3 (assay MGI:6383021).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Widespread |
3B
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3C
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Expression extending to peripheral retina.
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Specimen
3E/F:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gli1; green - Tubb3 (assay MGI:6383021).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
3F
,
3E
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Expression is centrally restricted. Expression boundary aligns with, or is slightly more centrally restricted than, boundary for acetylated Tubb3 expression.
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Specimen
3H/I:
embryonic day 12.5
(more )
Note: Double labeled: blue - Gli1; green - Tubb3 (assay MGI:6383021).
Specimen
3H/I:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gli1; green - Tubb3 (assay MGI:6383021).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
3H
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3I
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Expression is centrally restricted.
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Specimen
5A:
postnatal day 0
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina |
Strong |
Regionally restricted |
5A
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Abundant expression in retinal progenitor cells (RPCs).
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina |
Weak |
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5B
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Expression reduced compared with wild type.
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