Probe:	Cited2 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted		Expression was restricted to the cranial dorsal neural folds.
TS13: septum transversum	Present
TS13: neural ectoderm	Weak			Weak expression was detected in the neuroepithelium.
TS13: embryo mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Present
TS14: head mesenchyme	Present	Regionally restricted		Expression was detected in the mesenchyme around the telencephalon.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		4A
TS15: limb bud	Present		4A
TS15: optic vesicle	Present		4A
TS15: otocyst	Present		4A
TS15: telencephalic vesicle	Present		4A

Structure	Level	Pattern	Image	Note
TS15: somite	Present		4B
TS15: future brain	Present		4B	Expression was detected in the neuroepithelium in the cranial region.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		4C	Expression was pronounced in the cranial neural tube.
TS17: 1st branchial arch	Present		4C
TS17: 2nd branchial arch	Present		4C
TS17: limb	Present		4C
TS17: telencephalon	Present		4C

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		4D	Expression was detected in the neuroepithelium around the fore-, mid- and hindbrain.
TS17: hindbrain	Present		4D	Expression was detected in the neuroepithelium around the fore-, mid- and hindbrain.
TS17: midbrain	Present		4D	Expression was detected in the neuroepithelium around the fore-, mid- and hindbrain.
TS17: 1st branchial arch	Present		4D
TS17: neural tube	Present	Regionally restricted	4D	Expression was detected in the dorsal trunk neural tube.