Gene Expression Data
Assay Details
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Assay
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Reference:
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J:108393
Chung AC, et al., Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer. Dev Biol. 2006 May 1;293(1):13-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6360874
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more  )
Note: 1-2 somites
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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1-2 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
2A
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Authors report expression progressively restricted to anterior region of embryo.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nr6a1tm1Coo/Nr6a1tm1Coo
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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1-2 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
2B
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Expression in anterior region of embryo at onset of somitogenesis.
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Specimen
2C:
embryonic day 8.5
(more )
Note: 6 somites
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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6 somites
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nr6a1tm1Coo/Nr6a1tm1Coo
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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6 somites
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Specimen
2E:
embryonic day 9.5
(more )
Note: 25 somites
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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25 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
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2E
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Expression domain defines prospective forebrain and midbrain territory.
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| TS15: future midbrain |
Present |
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2E
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Expression domain defines prospective forebrain and midbrain territory.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nr6a1tm1Coo/Nr6a1tm1Coo
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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13 somites
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Analysis Tools