Reporter:	lacZ
	Direct Detection
Assay notes:	Authors used two lines Slain1<Gt(XG385)Byg> or Slain1<Gt(XG752)Byg>. Authors indicate that "a candidate primer approach was performed to characterise the exact site of transgene integration, which indicated that both XG385 and XG752 have the same insertion point, suggesting they are sister clones." The allele used in this assay was not detailed; annotations have been made to Slain1<Gt(XG385)Byg>.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Widespread	2B	Expression was detected throughout the entire epiblast.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Widespread	2C
TS15: tail	Present	Widespread	2C
TS15: branchial arch ectoderm	Present	Regionally restricted	2C	Expression was detected in the surface ectoderm of the branchial arches.
TS15: gut	Present		2C	In contrast to earlier in situ hybridization studies, beta-geo was also observed in the primitive gut tube.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		2E

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Widespread	2F
TS15: tail	Present	Widespread	2F

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		2G

Structure	Level	Pattern	Image	Note
TS19: eye	Strong		3B
TS19: forebrain	Strong		3B
TS19: neural tube	Strong		3B
TS19: gut	Weak		3B
TS19: oropharynx	Weak		3B

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present		3C
TS19: forelimb bud	Present		3C
TS19: hindlimb bud	Present		3C
TS19: limb mesenchyme	Present	Regionally restricted	3C	Expression was detected in the condensing mesenchyme of the developing limb buds.
TS19: 1st branchial arch	Present		3D , 3C
TS19: 1st branchial arch maxillary component	Present		3D , 3C
TS19: 2nd branchial arch	Present		3D , 3C
TS19: midgut loop	Present		3C , 3E

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Widespread
TS21: forelimb	Present		3G
TS21: hindlimb	Present		3G
TS21: forebrain	Strong		3G
TS21: midbrain	Strong		3G
TS21: spinal cord	Strong		3G

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb	Present		3H
TS21: midgut loop	Present		3H

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		3I

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		4A
TS19: central nervous system	Present		4A
TS19: facial ganglion	Present		4A
TS19: main bronchus	Present		4A
TS19: midgut loop lumen	Present		4A
TS19: nasal pit epithelium	Present		4A
TS19: optic stalk	Present		4A
TS19: otocyst	Present		4A
TS19: stomach lumen	Present		4A
TS19: trigeminal ganglion	Present		4A

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present		4B
TS21: eye	Present		4B
TS21: inferior vagus ganglion	Present		4B
TS21: main bronchus	Present		4B
TS21: renal tubule	Present		4B
TS21: trigeminal ganglion	Present		4B
TS21: vestibulo-cochlear ganglion	Present		4B

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		5C , 5A
TS19: forelimb bud mesenchyme	Strong	Regionally restricted	5C , 5A	Intense staining was detected in the distal region. Lower levels of expression were detected in the proximal region.
TS19: footplate apical ectodermal ridge	Present		5A , 5B

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit	Present	Non-Uniform	5D	The three central digital rays were marked by robust expression, whereas the two external digital rays had less intense staining.
TS21: future tarsus	Present	Regionally restricted	5D	Expression was detected in the proximal region.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present	Non-Uniform	5E	Expression outlined the three central digits, while the two external digital rays had less intense staining.
TS21: carpus pre-cartilage condensation	Present		5E
TS21: metacarpus pre-cartilage condensation	Present		5E
TS21: vibrissa	Present		5E

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		6A	Expression was detected in the neuroepithelium surrounding the telencephalic and mesencephalic ventricles.
TS15: telencephalon	Present		6A	Expression was detected in the neuroepithelium surrounding the telencephalic and mesencephalic ventricles.
TS15: head surface ectoderm	Present		6A
TS15: optic vesicle	Present		6A
TS15: neural crest	Present		6A	Expression was observed within condensations of neural crest cells located on either side of the mesencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS15: hindgut diverticulum	Present		6B
TS15: neural tube	Present		6B
TS15: neural crest	Present	Regionally restricted	6B	Expression was detected in the neural crest derived cells located on either side of the neural tube.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present		6C
TS19: midbrain	Present		6C
TS19: hindbrain	Present		6C	Expression was detected in the neuroepithelium surrounding the fourth and telencephalic ventricles. The level of expression varied between different stratified layers of the neuroepithelium.
TS19: telencephalon	Present		6C	Expression was detected in the neuroepithelium surrounding the fourth and telencephalic ventricles. The level of expression varied between different stratified layers of the neuroepithelium.
TS19: head mesenchyme	Present		6C
TS19: nasal pit	Present		6C
TS19: optic stalk	Present		6C
TS19: trigeminal ganglion	Present		6C
TS19: hindbrain ventricular layer	Present		6D , 6C
TS19: hindbrain roof plate	Absent		6C , 6D
TS19: telencephalon ventricular layer	Present		6C , 6E

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	6F	Expression was detected in the neuroepithelium surrounding the fourth and lateral ventricles. Staining was localised to the superficial layers of the neuroepithelium.
TS21: telencephalon	Present	Regionally restricted	6F	Expression was detected in the neuroepithelium surrounding the fourth and lateral ventricles. Staining was localised to the superficial layers of the neuroepithelium.
TS21: dorsal root ganglion	Present		6F
TS21: olfactory epithelium	Present		6F
TS21: choroid plexus	Absent		6F
TS21: remnant of Rathke's pouch	Absent		6F
TS21: tongue intrinsic muscle	Absent		6F

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		6G
TS21: spinal cord	Present		6G
TS21: segmental spinal nerve	Present	Regionally restricted	6G	Expression was detected in dorsal rootlets.
TS21: vertebral cartilage condensation	Absent		6G

Structure	Level	Pattern	Image	Note
TS21: hindbrain ventricular layer	Present	Regionally restricted	6H	Expression was detected in the ependymal layer.
TS21: hindbrain	Present	Regionally restricted	6H	Expression was localised to the superficial layer of the neuroepithelium surrounding the fourth ventricle.
TS21: trigeminal ganglion	Present		6H	Staining was detected in both the neurons and in the neural projections emanating from the trigeminal and vestibulocochlear ganglion towards the hindbrain.
TS21: vestibulo-cochlear ganglion	Present		6H	Staining was detected in both the neurons and in the neural projections emanating from the trigeminal and vestibulocochlear ganglion towards the hindbrain.
TS21: cochlea epithelium	Present	Non-Uniform	6H	Expression was detected throughout the cochlea epithelial layer, with the most pronounced expression at the apical surface.

Structure	Level	Pattern	Image	Note
TS21: lens	Present	Regionally restricted	6I	Expression was detected in the elongated cells of the posterior wall of the lens vesicle, but was not observed in the anterior wall.
TS21: neural retina	Present	Regionally restricted	6I	Expression was detected in the posterior side of the neural layer.
TS21: lower eyelid	Absent		6I
TS21: pigmented retinal epithelium	Absent		6I