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Gene Expression Data
Assay Details
Assay
Reference: J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6358503
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: The antibody recognized the cleaved form of the protein.
Results
Specimen 5A: embryonic day 10.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: dermomyotome Present 5A
Specimen 5B: embryonic day 10.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: red - Casp3; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 5B Cell death increased at the ventral lateral lip of the dermomyotome of the conditional knockout using the Pax3 Cre driver.
TS17: limb bud Ambiguous Regionally restricted 5B There was no increased apoptosis in muscle cells that migrated into the limb bud.
Specimen 5H: embryonic day 10.5; Smotm2Amc/Smotm2Amc, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 5H There was no evidence of ectopic or increased cell death in the ventral lateral lip of the dermomyotome of the conditional knockout using the Myf5 Cre driver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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