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Gene Expression Data
Assay Details
Assay
Reference: J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type: RNA in situ
MGI Accession ID: MGI:6358455
Gene symbol: Pax3
Gene name: paired box 3
Probe: Pax3 probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3A: embryonic day 10.5; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3A Expression was detected in muscle progenitor cells.
Specimen 3B: embryonic day 10.5; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3B Expression in conditional knockout muscle progenitor cells was comparable to wild type.
Specimen 3G: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3G Expression was detected in muscle progenitor cells.
Specimen 3H: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3H Expression in conditional knockout muscle progenitor cells was comparable to wild type.
Specimen 4E: embryonic day 11.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate Present Regionally restricted 4E Expression was detected in the distal limb bud.
Specimen 4F: embryonic day 11.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS19: handplate Absent 4F Expression was lost in the ventral autopod of the conditional knockout.
Specimen S4A: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4A Expression was detected in muscle progenitor cells.
Specimen S4B: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4B Expression was detected in muscle progenitor cells, as in wild type control.
Specimen S4G: embryonic day 11.0; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4G Expression was detected in muscle progenitor cells.
Specimen S4H: embryonic day 11.0; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4H Expression was detected in muscle progenitor cells, as in wild type control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory