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Gene Expression Data
Assay Details
Assay
Reference: J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type: RNA in situ
MGI Accession ID: MGI:6358452
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Probe: Myod1 probe15
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS21: forelimb muscle Present 1A
Specimen 1B: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb muscle Present 1B
Specimen 1C: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS21: forelimb muscle Present 1C The expression domain appeared to be reduced in size at E12.5.
Specimen 3M: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3M Expression was detected in forelimb ventral and dorsal muscle cells.
Specimen 3N: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3N At E10.75, expression was delayed in the conditional knockout ventral muscle cells. Expression in dorsal muscle cells was largely unaffected.
Specimen 3Q: embryonic day 11.0; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS18: forelimb bud Present Regionally restricted 3Q Expression was detected in forelimb ventral and dorsal muscle cells.
Specimen 3R: embryonic day 11.0; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS18: forelimb bud Present Regionally restricted 3R Expression in forelimb ventral muscle cells had recovered by E11 in the conditional knockout.
Specimen 4A: embryonic day 11.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate Present Regionally restricted 4A Expression was detected in myogenic cells in the ventral autopod.
Specimen 4B: embryonic day 11.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS19: handplate Absent 4B Expression was absent in the distal forelimb.
Specimen 4C: embryonic day 12.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 4C Expression was detected in myogenic cells in the ventral autopod.
Specimen 4D: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS20: hand Absent 4D Expression was absent in the distal forelimb.
Specimen 5I: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: limb Present Regionally restricted 5I Expression was detected in distal myocytes.
Specimen 5J: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: limb Present Regionally restricted 5J In the conditional knockout using the Myf5 Cre driver, expression didn't extend as far distally as in wild type.
Specimen 7K: embryonic day 12.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb muscle Present Regionally restricted 7K
Specimen 7L: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS21: forelimb muscle Present Regionally restricted 7L In the conditional knockout mutant, expression didn't extend as far distally in the forelimb muscle as in wild type.
Specimen S1A: embryonic day 12.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: somite Present S1A
Specimen S1B: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS20: somite Present Regionally restricted S1B In the conditional knockout mutant, expression was lost in the cervical somites.
Specimen S1E: embryonic day 13.5; Smotm2Amc/Smo+, Tcf7l2tm1(EGFP/cre)Mrc/Tcf7l2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: limb muscle Present S1E
Specimen S1F: embryonic day 13.5; Smotm2Amc/Smotm2Amc, Tcf7l2tm1(EGFP/cre)Mrc/Tcf7l2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: limb muscle Present S1F Expression in the conditional knockout mutant using the Tcf4-Cre driver appeared normal.
Specimen S4M: embryonic day 11.0; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4M Expression was detected in hindlimb ventral and dorsal muscle cells.
Specimen S4N: embryonic day 11.0; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS18: hindlimb bud Absent S4N At E11, expression was delayed in the conditional knockout ventral and dorsal muscle cells.
Specimen S4O: embryonic day 11.25; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Structure Level Pattern Image Note
TS19: hindlimb bud Present Regionally restricted S4O Expression was detected in hindlimb ventral and dorsal muscle cells.
Specimen S4P: embryonic day 11.25; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS19: hindlimb bud Present Regionally restricted S4P Expression in hindlimb ventral and dorsal muscle cells had recovered by E11.25 in the conditional knockout.
Specimen S5A: embryonic day 12.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: foot Present Regionally restricted S5A Expression was detected in myogenic cells in the ventral hindlimb.
Specimen S5B: embryonic day 12.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS20: foot Weak Regionally restricted S5B There was a reduced myogenic population in the conditional knockout mutant hindlimb autopod.
Specimen S7E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: autopod Present Regionally restricted S7E Expression was detected in autopod muscles.
Specimen S7F: embryonic day 13.5; Smotm2Amc/Smotm2Amc, Tg(MYOD1-cre)M23Glh/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: autopod Present Regionally restricted S7F Expression was truncated distally in conditional knockout autopod muscles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory