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Gene Expression Data
Assay Details
Assay
Reference: J:104611 Moore-Scott BA, et al., Differential expression of Sonic hedgehog along the anterior-posterior axis regulates patterning of pharyngeal pouch endoderm and pharyngeal endoderm-derived organs. Dev Biol. 2005 Feb 15;278(2):323-35
Assay type: RNA in situ
MGI Accession ID: MGI:6356718
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube floor plate Strong 1A
TS15: notochord Strong 1A
TS15: pharynx epithelium Present Regionally restricted 1A Expression in both dorsal and ventral pharygeal endoderm.
TS15: 1st branchial pouch endoderm Absent 1A
Specimen 1B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube floor plate Strong 1B
TS15: notochord Strong 1B
TS15: pharynx epithelium Present Regionally restricted 1B Expression in both dorsal and ventral pharygeal endoderm.
TS15: thyroid primordium endoderm Absent 1B Ventral endoderm expression excluded in thyroid diverticulum.
TS15: 2nd branchial pouch endoderm Absent 1B
Specimen 1C: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube floor plate Strong 1C
TS15: notochord Strong 1C
TS15: pharynx epithelium Present Regionally restricted 1C Expression in both dorsal and ventral pharygeal endoderm.
TS15: 3rd branchial pouch endoderm Absent 1C
Specimen 1D: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube floor plate Strong 1D
TS15: notochord Strong 1D
TS15: pharynx epithelium Present Regionally restricted 1D Expression in both dorsal and ventral pharygeal endoderm. Expression throughout pharyngeal endoderm at location where the fourth pouch will form, just below the third pouch and above the future laryngeo/tracheal groove.
Specimen 1I: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pharynx epithelium Strong 1I
TS17: 1st branchial pouch Present Regionally restricted 1I Expression expanded furtherinto first pouch.
Specimen 1J: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pharynx epithelium Strong 1J
TS17: 2nd branchial pouch Present Regionally restricted 1J Expression expanded further into second pouch.
Specimen 1K: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pharynx epithelium Strong 1K
TS17: 3rd branchial pouch Present Regionally restricted 1K Expression remains restricted to opening of third pouch.
Specimen 1L: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pharynx epithelium Strong 1L
TS17: 4th branchial pouch Present Regionally restricted 1L Expression remains restricted to opening of fourth pouch.
Specimen 1Q: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial pouch Present Regionally restricted 1Q Expression expanded further into first pouch, although still excluded from distal tips.
Specimen 1R: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 2nd branchial pouch Present Regionally restricted 1R Expression expanded further into second pouch, although still excluded from distal tips.
Specimen 1S: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 3rd branchial pouch Absent 1S
Specimen 1T: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 4th branchial pouch Absent 1T
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory