Nodal RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93140 Lu CC, et al., Multiple roles for Nodal in the epiblast of the mouse embryo in the establishment of anterior-posterior patterning. Dev Biol. 2004 Sep 1;273(1):149-59
Assay type:	RNA in situ
MGI Accession ID:	MGI:6342934
Gene symbol:	Nodal
Gene name:	nodal growth differentiation factor

Probe:	Nodal probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2B wt ©

Specimen 2B wt: embryonic day 6.0 (more

)

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Regionally restricted	2B wt	Expression in posterior epiblast.
TS8: primitive endoderm	Present		2B wt	Expression in visceral endoderm.

2B m ©

Specimen 2B m: embryonic day 6.25; Nodal^tm1Rob/Nodal^tm5Rob, Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Absent		2B m
TS8: primitive endoderm	Absent		2B m

3D wt ©

Specimen 3D wt: embryonic day 6.5 (more

)

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		3D wt

3D m ©

Specimen 3D m: embryonic day 6.5; Meox2^tm1(cre)Sor/Meox2⁺, Nodal^tm1Rob/Nodal^tm5Rob (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Weak		3D m	Reduced levels of expression in epiblast compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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