Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74069
Morris-Wiman J, et al., Temporospatial distribution of matrix metalloproteinase and tissue inhibitors of matrix metalloproteinases during murine secondary palate morphogenesis. Anat Embryol (Berl). 2000 Aug;202(2):129-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6342631
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Gene symbol:
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Timp2
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Gene name:
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tissue inhibitor of metalloproteinase 2
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Antibody:
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anti-Timp-2
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Detection system:
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fluorescein
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Assay notes:
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Fixation with acid alcohol.
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Results
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Specimen
3D:
embryonic day 12.0
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf mesenchyme |
Present |
Regionally restricted |
3D
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Expression within maxillary and central regions of palatal mesenchyme. No expression within nasal mesenchyme.
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| TS20: palatal shelf epithelium |
Present |
Regionally restricted |
3D
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Expression within palatal epithelial basement membrane.
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Specimen
3E:
embryonic day 13.0
(more )
Specimen
3E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Very strong |
Widespread |
3E
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Expression greatly intensified within all regions of palatal mesenchyme, most profound within nasal, dorsal medial, central and maxillary mesenchyme. Expression relatively decreased but detected within ventral and mid-oral mesenchyme.
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| TS21: tooth mesenchyme |
Absent |
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3E
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No expression in lateral or tooth germ mesenchyme.
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| TS21: palatal shelf epithelium |
Present |
Regionally restricted |
3E
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Expression in palatal basement membrane, increased within nasal and oral regions.
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Specimen
3F:
embryonic day 14.0
(more )
Specimen
3F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Present |
Regionally restricted |
3F
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Expression disappeared from within nasal mesenchyme and associated osteogenic sites. Intense expression remained within dorsal medial mesenchyme, extending ventrally to outline the regressing palatal seam. Band of expression through maxillary and central mesenchyme. Decreased but detectable expression within ventral medial mesenchyme.
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Specimen
gd 14-fused not shown:
embryonic day 14.0
(more )
Specimen
gd 14-fused not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Weak |
Regionally restricted |
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Expression reduced in all mesenchymal regions exceptor the dorsal medial.
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Analysis Tools