Gene Expression Data
Assay Details
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Assay
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Reference:
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J:91443
Wilm B, et al., The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate. Dev Biol. 2004 Jul 1;271(1):176-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6342074
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 8.75
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Present |
Widespread |
5A
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Expression throughout somite.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Foxc2tm1Blh/Foxc2tm1Blh
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Weak |
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5B
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Weaker expression in somites than in wildtype, and onset of expression is delayed.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1+
Foxc2tm1Blh/Foxc2tm1Blh
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Trace |
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5C
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Expression is strongly reduced - almost completely lost.
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Specimen
5G:
embryonic day 8.75
(more )
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: dermomyotome |
Strong |
Regionally restricted |
5G
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Strongest expression in medial lip of differentiating dermomyotome.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Foxc2tm1Blh/Foxc2tm1Blh
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: dermomyotome |
Weak |
Regionally restricted |
5H
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Weaker expression at medial dermomyotomal lip. expression is delayed.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1+
Foxc2tm1Blh/Foxc2tm1Blh
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: dermomyotome |
Trace |
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5I
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Expression is strongly reduced - almost completely lost.
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Analysis Tools
