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Gene Expression Data
Assay Details
Assay
Reference: J:241573 Pui HP, et al., Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mech Dev. 2017 Apr;144(Pt B):125-139
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6305359
Gene symbol: Cdh1
Gene name: cadherin 1
Results
Specimen 1B E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364).
Structure Level Pattern Image Note
TS25: germ cell of testis Present 1B E17.5 PLZF+ECAD Expression was detected in both the gonocytes and nascent spermatogonia.

Specimen 1B E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 1B E18.5 PLZF+ECAD Expression was detected in both the gonocytes and nascent spermatogonia.

Specimen 1B P0.5: postnatal day 0.5 (more )
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364).
Structure Level Pattern Image Note
TS27: germ cell of testis Present 1B P0.5 PLZF+ECAD Expression was detected in both the gonocytes and nascent spermatogonia.

Specimen 1B P1.5: postnatal day 1.5 (more )
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364).
Structure Level Pattern Image Note
TS27: germ cell of testis Present 1B P1.5 PLZF+ECAD Expression was detected in both the gonocytes and nascent spermatogonia.

Specimen 2B E17.5: embryonic day 17.5 (more )
Note: Triple labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364); white - Laminin.
Structure Level Pattern Image Note
TS25: germ cell of testis Present 2B E17.5 PLZF+ECAD+Laminin Expression was detected in the cell membrane of gonocytes. A small number had relocated.

Specimen 2B E18.5: embryonic day 18.5 (more )
Note: Triple labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364); white - Laminin.
Structure Level Pattern Image Note
TS26: germ cell of testis Present 2B E18.5 PLZF+ECAD+Laminin Expression was detected in the cell membrane of gonocytes. Relocated gonocytes were noted.

Specimen 2B P0.5: postnatal day 0.5 (more )
Note: Triple labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 2B P0.5 PLZF+ECAD+Laminin Expression was detected in the cell membrane of gonocytes. Relocated gonocytes were noted.

Specimen 2B P1.5: postnatal day 1.5 (more )
Note: Triple labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 2B P1.5 PLZF+ECAD+Laminin Expression was detected in the cell membrane of gonocytes. Relocated gonocytes were noted.

Specimen 3A E17.5: embryonic day 17.5 (more )
Note: Triple labeled: red - Cdh1; green - Foxo1 (assay MGI:6306311); white - Laminin.
Structure Level Pattern Image Note
TS25: germ cell of testis Present 3A E17.5 FOXO1+ECAD+Laminin

Specimen 3A E18.5: embryonic day 18.5 (more )
Note: Triple labeled: red - Cdh1; green - Foxo1 (assay MGI:6306311); white - Laminin.
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3A E18.5 FOXO1+ECAD+Laminin

Specimen 3A P0.5: postnatal day 0.5 (more )
Note: Triple labeled: red - Cdh1; green - Foxo1 (assay MGI:6306311); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3A P0.5 FOXO1+ECAD+Laminin

Specimen 3A P1.5: postnatal day 1.5 (more )
Note: Triple labeled: red - Cdh1; green - Foxo1 (assay MGI:6306311); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3A P1.5 FOXO1+ECAD+Laminin

Specimen S1A E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh1; green - PH3.
Structure Level Pattern Image Note
TS25: germ cell of testis Present S1A E17.5

Specimen S1A E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Cdh1; green - PH3.
Structure Level Pattern Image Note
TS26: germ cell of testis Present S1A E18.5

Specimen S1A P0.5: postnatal day 0.5 (more )
Note: Double labeled: red - Cdh1; green - PH3.
Structure Level Pattern Image Note
TS27: germ cell of testis Present S1A P0.5 A small subset of gonocytes had reinitiated the cell cycle.

Specimen S1A P1.5: postnatal day 1.5 (more )
Note: Double labeled: red - Cdh1; green - PH3.
Structure Level Pattern Image Note
TS27: germ cell of testis Present S1A P1.5 Mitotic germ cells were noted at P1.5.

Specimen S1C: postnatal day 1.5 (more )
Note: Triple labeled: red - Cdh1; green - Zbtb16 (assay MGI:6305364); white - PH3.
Structure Level Pattern Image Note
TS27: germ cell of testis Present S1C P1.5 top

Specimen S3A E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh1; green - Mapk1 (assay MGI:6306317) and Mapk3 (assay MGI:6306318).
Structure Level Pattern Image Note
TS25: germ cell of testis Present S3A E17.5 pERK+ECAD+Dapi

Specimen S3A E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Cdh1; green - Mapk1 (assay MGI:6306317) and Mapk3 (assay MGI:6306318).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S3A E18.5 pERK+ECAD+Dapi

Specimen S3A P0.5: postnatal day 0.5 (more )
Note: Double labeled: red - Cdh1; green - Mapk1 (assay MGI:6306317) and Mapk3 (assay MGI:6306318).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S3A P0.5 pERK+ECAD+Dapi

Specimen S3A P1.5: postnatal day 1.5 (more )
Note: Double labeled: red - Cdh1; green - Mapk1 (assay MGI:6306317) and Mapk3 (assay MGI:6306318).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S3A P1.5 pERK+ECAD+Dapi

Specimen S3B E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh1; green - Rps6 (assay MGI:6306319).
Structure Level Pattern Image Note
TS25: germ cell of testis Present S3B E17.5 pS6+ECAD+Dapi

Specimen S3B E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Cdh1; green - Rps6 (assay MGI:6306319).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S3B E18.5 pS6+ECAD+Dapi

Specimen S3B P0.5: postnatal day 0.5 (more )
Note: Double labeled: red - Cdh1; green - Rps6 (assay MGI:6306319).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S3B P0.5 pS6+ECAD+Dapi

Specimen S3B P1.5: postnatal day 1.5 (more )
Note: Double labeled: red - Cdh1; green - Rps6 (assay MGI:6306319).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S3B P1.5 pS6+ECAD+Dapi


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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory