About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296597
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved caspase-3
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 6A 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS26: testis Absent 6A 18.5 dpc CT No expressing cells in control testes.
Specimen 6A 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: testis Present 6A 18.5 dpc KO Authors report significant number of expressing cells starting at 18.5 dpc.
Specimen 6A P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS27: testis Absent 6A P0 CT No expressing cells in control testes.
Specimen 6A P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: testis Strong 6A P0 KO Prominent number of expressing cells.
Specimen 6A P2 CT: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS27: testis Absent 6A P2 CT No expressing cells in control testes.
Specimen 6A P2 KO: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: testis Strong 6A P2 KO Prominent number of expressing cells.
Specimen 6A P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS28: testis Absent 6A P5 CT No expressing cells in control testes.
Specimen 6A P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: testis Ambiguous 6A P5 KO
Specimen 6B: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Present Regionally restricted 6B Expression in Sertoli cells inside testis cords.
TS27: Sertoli cell Sertoli cell Present Regionally restricted 6B Expression in Sertoli cells inside testis cords.
Specimen 6C: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: germ cell of testis Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
TS27: seminiferous cord Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
TS27: Sertoli cell Sertoli cell Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
Specimen 6D: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS27: interstitium of the testis Present 6D Expression in cells in the interstitium.
Specimen 6E: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS27: interstitium of the testis Weak 6E Only a few expressing cells in the interstitium.
Specimen 7C CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - Casp3; red - Hsd3b (assay MGI:6296592); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Absent 7C CT
Specimen 7C KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - Hsd3b (assay MGI:6296592); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Absent 7C KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory