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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296594
Gene symbol: Cyp21a1
Gene name: cytochrome P450, family 21, subfamily a, polypeptide 1
Antibody: anti-CYP21
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 2 14.5 dpc CT: embryonic day 14.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS22: adrenal cortex Present 2_14.5 dpc CT
Specimen 2 14.5 dpc KO: embryonic day 14.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS22: adrenal cortex Present 2_14.5 dpc KO
Specimen 2 16.5 dpc CT: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 2_16.5 dpc CT
Specimen 2 16.5 dpc KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 2_16.5 dpc KO
Specimen 2 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 2_18.5 dpc CT
Specimen 2 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Weak 2_18.5 dpc KO
Specimen 2 P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Present 2_P0 CT
Specimen 2 P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Weak 2_P0 KO
Specimen 2 P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Present 2_P5 CT
Specimen 2 P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Cyp21a1; green - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Trace 2_P5 KO Authors state almost all cortical cells were lost at this stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory