Gene Expression Data
Assay Details
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Assay
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Reference:
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J:185807
Nowotschin S, et al., Interaction of Wnt3a, Msgn1 and Tbx6 in neural versus paraxial mesoderm lineage commitment and paraxial mesoderm differentiation in the mouse embryo. Dev Biol. 2012 Jul 1;367(1):1-14
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6287742
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Gene symbol:
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Tbx6
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Gene name:
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T-box 6
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Probe:
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Tbx6 cDNA1
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Probe preparation:
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Antisense RNA
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Results
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Specimen
2G:
embryonic day 7.0
(more  )
Note: early bud stage
Specimen
2G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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early bud stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
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2G
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Expression restricted to posterior side of embryo in vicinity of primitive streak.
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Specimen
2H:
embryonic day 7.5
(more )
Note: late bud stage
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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late bud stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
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2H
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Expression restricted to posterior side of embryo in vicinity of primitive streak.
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Specimen
2I1:
embryonic day 7.5
(more )
Note: head fold stage
Specimen
2I1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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head fold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2I1
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Expression predominantly in medial-lateral domain (wings) within the nascent mesoderm.
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| TS11: primitive streak |
Present |
Regionally restricted |
2I1
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Expression in a few epiblast cells in the site of cell ingression.
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Specimen
2I2:
embryonic day 7.5
(more )
Note: head fold stage
Specimen
2I2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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head fold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2I2
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Expression predominantly in medial-lateral domain (wings) within the nascent mesoderm.
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| TS11: primitive streak |
Present |
Regionally restricted |
2I2
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Expression in a few epiblast cells in the site of cell ingression.
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Specimen
2I3:
embryonic day 7.5
(more )
Note: head fold stage
Specimen
2I3:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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head fold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2I3
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Expression extended laterally from streak into presomitic mesoderm.
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| TS11: primitive streak |
Present |
Regionally restricted |
2I3
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Expression in a few epiblast cells in the site of cell ingression.
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Specimen
2I4:
embryonic day 7.5
(more )
Note: head fold stage
Specimen
2I4:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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head fold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2I4
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Expression extended laterally from streak into presomitic mesoderm.
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| TS11: primitive streak |
Present |
Regionally restricted |
2I4
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Expression in a few epiblast cells in the site of cell ingression.
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Specimen
2P1:
embryonic day 8.0
(more )
Note: 3-4 somite stage
Specimen
2P1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-4 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Absent |
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2P1
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Expression ceased in epiblast cells coincident with the start of somite segmentation.
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| TS12: unsegmented mesenchyme |
Present |
Regionally restricted |
2P1
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In caudal regions expression in entire mesoderm cell population, close to lateral epiblast of the tail region.
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Specimen
2P2:
embryonic day 8.0
(more )
Note: 3-4 somite stage
Specimen
2P2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-4 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: unsegmented mesenchyme |
Present |
Regionally restricted |
2P2
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In caudal regions expression in entire mesoderm cell population, close to lateral epiblast of the tail region.
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Specimen
2Q1:
embryonic day 8.5
(more )
Note: 8-9 somite stage
Specimen
2Q1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-9 somite stage
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Specimen
2Q2:
embryonic day 8.5
(more )
Note: 8-9 somite stage
Specimen
2Q2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-9 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: unsegmented mesenchyme |
Present |
Regionally restricted |
2Q2
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Expression ends abruptly at rostral border where somites segment.
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Specimen
2Q3:
embryonic day 8.5
(more )
Note: 8-9 somite stage
Specimen
2Q3:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-9 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: unsegmented mesenchyme |
Present |
Regionally restricted |
2Q3
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In rostral part of presomitic mesenchyme, dorsal expression adjacent to neural plate.
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Specimen
2R1:
embryonic day 9.0
(more )
Note: 19 somite stage
Specimen
2R1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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19 somite stage
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Specimen
2R2:
embryonic day 9.0
(more )
Note: 19 somite stage
Specimen
2R2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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19 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: unsegmented mesenchyme |
Present |
Regionally restricted |
2R2
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Expression ends abruptly at rostral border where somites segment.
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Specimen
2R3:
embryonic day 9.0
(more )
Note: 19 somite stage
Specimen
2R3:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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19 somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: unsegmented mesenchyme |
Present |
Regionally restricted |
2R3
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In caudal regions expression in entire mesoderm cell population, close to lateral epiblast of the tail region.
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Specimen
5C1:
embryonic day 8.5
(more )
Note: 10-12 somite stage
Specimen
5C1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5C2:
embryonic day 8.5
(more )
Note: 10-12 somite stage
Specimen
5C2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5E1:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5E2:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5G1:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Msgn1tm1Wb/Msgn1tm1Wb
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5G2:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Msgn1tm1Wb/Msgn1tm1Wb
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5L1:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Msgn1tm1Wb/Msgn1tm1Wb
Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
5L2:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Msgn1tm1Wb/Msgn1tm1Wb
Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-12 somite stage
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Specimen
S3C:
embryonic day 7.5
(more )
Note: head fold stage
Specimen
S3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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|
Note:
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head fold stage
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Specimen
S3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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head fold stage
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Specimen
S3G:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Msgn1tm1Wb/Msgn1tm1Wb
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Age:
|
embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
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|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
head fold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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S3G
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Expression was not down regulated at these early stages.
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Specimen
S3L:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
|
Msgn1tm1Wb/Msgn1tm1Wb
Wnt3atm1Amc/Wnt3atm1Amc
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|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
head fold stage
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
|
S3L
|
Expression was not down regulated at these early stages.
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Analysis Tools