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Gene Expression Data
Assay Details
Assay
Reference: J:266538 Backer S, et al., Trio GEF mediates RhoA activation downstream of Slit2 and coordinates telencephalic wiring. Development. 2018 Oct 2;145(19):dev153692
Assay type: Western blot
MGI Accession ID: MGI:6286057
Gene symbol: Rhoa
Gene name: ras homolog family member A
Antibody: anti-Rho A (26C4)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Western blot was performed after pull-down assays using GST-Rhotekin to test the activated form.
Results
Image: 1B right ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
ctl E14.5 TS22: telencephalon Present involves: BALB/c Not Specified ventral telencephalon
Trio-/- E14.5 TS22: telencephalon Present (a) involves: 129S4/SvJae * BALB/c Triotm1Mst/Triotm1MstNot Specified ventral telencephalon
Notes:
(a) A greater than twofold decrease in activated/total Rhoa ratio in mutant compared with WT extracts.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory