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Gene Expression Data
Assay Details
Assay
Reference: J:119781 Vreugdenhil E, et al., Doublecortin-like, a microtubule-associated protein expressed in radial glia, is crucial for neuronal precursor division and radial process stability. Eur J Neurosci. 2007 Feb;25(3):635-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6267029
Gene symbol: Dcx
Gene name: doublecortin
Antibody: anti-doublecortin (C-18, sc-8066)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6C: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: telencephalon ventricular layer Absent 6C Mitotic cells at the ventricular surface were immunonegative.

Specimen 6E: embryonic day 11.0 (more )
Note: Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 6E Dcx-immunopositive processes were in close apposition to, but not overlapping with, the mitotic Dclk1-immunopositive cells.

Specimen 6F: embryonic day 11.0 (more )
Note: Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 6F Dcx-immunopositive processes were in close apposition to, but not overlapping with, the mitotic Dclk1-immunopositive cells.

Specimen 6K: embryonic day 11.0 (more )
Note: Triple labeled: green/white - Dcx; red/white - Vim (assay MGI:6267040); red/white - Dclk1 (assay MGI:6267023).
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 6K Expression was detected in the second proliferative layer between the subventricular zone and primitive plexiform zone, which included cells that were triple-labeled for Dclk1, Vim, and Dcx.

Specimen 6L: embryonic day 11.0 (more )
Note: Double labeled: yellow - Dcx; red - Dclk1 (assay MGI:6267023).
Structure Level Pattern Image Note
TS18: future spinal cord Present Regionally restricted 6L , 6M Expression was non-overlapping with Dclk1-immunolabeling.

Specimen 6N: embryonic day 11.0 (more )
Note: Double labeled: green - Dcx; purple - Vim (assay MGI:6267040).
Structure Level Pattern Image Note
TS18: neural tube Present Regionally restricted 6N Expression was not found in the Vim-immunopositive radial glia. Only rarely was co-labeling seen.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory