Gene Expression Data
Assay Details
Assay
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Reference:
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J:119781
Vreugdenhil E, et al., Doublecortin-like, a microtubule-associated protein expressed in radial glia, is crucial for neuronal precursor division and radial process stability. Eur J Neurosci. 2007 Feb;25(3):635-48
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6267029
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Gene symbol:
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Dcx
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Gene name:
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doublecortin
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Results
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Specimen
6C:
embryonic day 10.0
(more )
Specimen
6C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6E:
embryonic day 11.0
(more )
Note: Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
Specimen
6E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
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Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
6E
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Dcx-immunopositive processes were in close apposition to, but not overlapping with, the mitotic Dclk1-immunopositive cells.
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Specimen
6F:
embryonic day 11.0
(more )
Note: Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Dcx; red - Dclk1 (assay MGI:6267023).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
6F
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Dcx-immunopositive processes were in close apposition to, but not overlapping with, the mitotic Dclk1-immunopositive cells.
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Specimen
6K:
embryonic day 11.0
(more )
Note: Triple labeled: green/white - Dcx; red/white - Vim (assay MGI:6267040); red/white - Dclk1 (assay MGI:6267023).
Specimen
6K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green/white - Dcx; red/white - Vim (assay MGI:6267040); red/white - Dclk1 (assay MGI:6267023).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
6K
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Expression was detected in the second proliferative layer between the subventricular zone and primitive plexiform zone, which included cells that were triple-labeled for Dclk1, Vim, and Dcx.
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Specimen
6L:
embryonic day 11.0
(more )
Note: Double labeled: yellow - Dcx; red - Dclk1 (assay MGI:6267023).
Specimen
6L:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: yellow - Dcx; red - Dclk1 (assay MGI:6267023).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
6L
,
6M
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Expression was non-overlapping with Dclk1-immunolabeling.
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Specimen
6N:
embryonic day 11.0
(more )
Note: Double labeled: green - Dcx; purple - Vim (assay MGI:6267040).
Specimen
6N:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Dcx; purple - Vim (assay MGI:6267040).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: neural tube |
Present |
Regionally restricted |
6N
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Expression was not found in the Vim-immunopositive radial glia. Only rarely was co-labeling seen.
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