Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119781
Vreugdenhil E, et al., Doublecortin-like, a microtubule-associated protein expressed in radial glia, is crucial for neuronal precursor division and radial process stability. Eur J Neurosci. 2007 Feb;25(3):635-48
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6267023
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Gene symbol:
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Dclk1
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Gene name:
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doublecortin-like kinase 1
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Antibody:
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anti-DCL
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
6D:
embryonic day 10.0
(more  )
Specimen
6D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: telencephalon ventricular layer |
Present |
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6D
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6A
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6B
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Expression was in mitotic cells at the ventricular surface. Immunostaining was associated with both kinetochore microtubules and polar microtubules.
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Specimen
6E:
embryonic day 11.0
(more )
Note: Double labeled: red - Dclk1; green - Dcx (assay MGI:6267029).
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Dclk1; green - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
6E
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Many immunopositive precursor cells were found in the higher regions of the subventricular zone-intermediate zone. These cells were Dcx-negative.
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Specimen
6F:
embryonic day 11.0
(more )
Note: Double labeled: red - Dclk1; green - Dcx (assay MGI:6267029).
Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Dclk1; green - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
6F
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Many immunopositive precursor cells were found in the higher regions of the subventricular zone-intermediate zone. These cells were Dcx-negative.
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Specimen
6I:
embryonic day 13.0
(more )
Note: Double labeled: red - Dclk1; purple - Vim (assay MGI:6267040).
Specimen
6I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Dclk1; purple - Vim (assay MGI:6267040).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon ventricular layer |
Present |
Regionally restricted |
6I
,
6J
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Immunolabeling was noted in the neuroepithelial layer of the ventricular zone in Vim-immunopositive radial glia and neural precursors.
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Specimen
6K:
embryonic day 11.0
(more )
Note: Triple labeled: red/white - Dclk1; red/white - Vim (assay MGI:6267040); green/white - Dcx (assay MGI:6267029).
Specimen
6K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red/white - Dclk1; red/white - Vim (assay MGI:6267040); green/white - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
6K
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Expression was detected in Vim-immunopositive radial glial cells throughout the neuroepithelium and ventricular zone and in neuroblasts in the subventricular zone-intermediate zone. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
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| TS18: telencephalon ventricular layer |
Present |
Regionally restricted |
6K
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Expression was detected in Vim-immunopositive radial glial cells throughout the neuroepithelium and ventricular zone and in neuroblasts in the subventricular zone-intermediate zone. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
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Specimen
6L:
embryonic day 11.0
(more )
Note: Double labeled: red - Dclk1; yellow - Dcx (assay MGI:6267029).
Specimen
6L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Dclk1; yellow - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
6L
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6M
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Expression was detected at upper and lower border regions. Immunopositive long radial glia-like fibers extended radially amid extensive (non-overlapping) Dcx expression.
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