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Gene Expression Data
Assay Details
Assay
Reference: J:194052 Corbel C, et al., Unusual chromatin status and organization of the inactive X chromosome in murine trophoblast giant cells. Development. 2013 Feb;140(4):861-72
Assay type: RNA in situ
MGI Accession ID: MGI:6259648
Gene symbol: Huwe1
Gene name: HECT, UBA and WWE domain containing 1
Results
Specimen 1E,F: embryonic day 7.0 (more )
Note: Double labeled: magenta - Huwe1; green - Xist (assay MGI:6259643).
Structure Level Pattern Image Note
TS10: extraembryonic visceral endoderm Present 1F Huwe1 VE , 1F Merge VE , 1E Monoallelic expression was detected on the active X.
TS10: trophoblast giant cell Present 1F Merge TGC , 1F Huwe1 TGC , 1E Biallelic expression was detected on both the inactive and active X.

Specimen not shown: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo Present Expression was detected from the active X in the majority of cells in all lineages at this stage.
TS10: extraembryonic component Present Expression was detected from the active X in the majority of cells in all lineages at this stage.

Specimen Table 1: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: trophoblast giant cell Present Over 30% of trophoblast giant cells showed some degree of biallelic expression at this stage.

Specimen 2B: embryonic day 8.0 (more )
Note: Double labeled: magenta - Huwe1; green - Xist (assay MGI:6259643); red - Mecp2 (assay MGI:6259661).
Structure Level Pattern Image Note
TS12: trophoblast giant cell Present 2B Huwe1 , 2B Merge Monoallelic expression was detected.

Specimen Table S1: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted Expression from the active X was detected.
TS10: extraembryonic visceral endoderm Present Regionally restricted Expression from the active X was detected.
TS10: trophoblast giant cell Present Regionally restricted Expression from the active X was detected.
TS10: extraembryonic component Present Expression from the active X was detected in the ectoplacental cone and chorion.

Specimen Table S1: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: chorion Present Expression from the active X was detected.
TS11: ectoplacental cone Present Expression from the active X was detected.
TS11: embryo Present Expression from the active X was detected.
TS11: extraembryonic visceral endoderm Present Expression from the active X was detected.
TS11: trophoblast giant cell Present Expression from the active X was detected.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory