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Gene Expression Data
Assay Details
Assay
Reference: J:194052 Corbel C, et al., Unusual chromatin status and organization of the inactive X chromosome in murine trophoblast giant cells. Development. 2013 Feb;140(4):861-72
Assay type: RNA in situ
MGI Accession ID: MGI:6259643
Gene symbol: Xist
Gene name: inactive X specific transcripts
Results
Specimen 1B: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Kif4 (assay MGI:6259646).
Structure Level Pattern Image Note
TS10: extraembryonic component Present 1B Xist was detected in a single RNA domain in the overwhelming majority of cells.
Specimen 1C: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Kif4 (assay MGI:6259646).
Structure Level Pattern Image Note
TS10: embryo Present 1C Xist was detected in a single RNA domain in the overwhelming majority of cells.
Specimen 1E,F: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Huwe1 (assay MGI:6259648).
Structure Level Pattern Image Note
TS10: extraembryonic visceral endoderm Present 1E , 1F Xist VE , 1F Merge VE
TS10: trophoblast giant cell Present 1E , 1F Xist TGC , 1F Merge TGC
Specimen 2A: embryonic day 7.5 (more )
Note: Double labeled: green - Xist; red - Rlim (assay MGI:6259660).
Structure Level Pattern Image Note
TS11: trophoblast giant cell Present 2A Merge , 2A Xist
Specimen 2B: embryonic day 8.0 (more )
Note: Triple labeled: green - Xist; red - Mecp2 (assay MGI:6259661); magenta - Huwe1 (assay MGI:6259648).
Structure Level Pattern Image Note
TS12: trophoblast giant cell Present 2B Xist , 2B Merge
Specimen 2C: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Atrx (assay MGI:6259659).
Structure Level Pattern Image Note
TS10: trophoblast giant cell Present 2C Merge , 2C Xist
Specimen S1B: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Atrx (assay MGI:6259666).
Structure Level Pattern Image Note
TS10: extraembryonic component Present Regionally restricted S1B Xist , S1B Merge There was no overlap of the Xist expression domain with the Atrx protein in the two nuclei from chorion shown in the figure. Only 5.8% of chorion nuclei show partial overlap of expression.
Specimen S1C: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Atrx (assay MGI:6259666).
Structure Level Pattern Image Note
TS10: trophoblast giant cell Present S1C Xist , S1C Merge Expression of Xist partially overlaps with Atrx protein in 25% of trophoblast giant cells.
Specimen S1D: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Atrx (assay MGI:6259659).
Structure Level Pattern Image Note
TS10: extraembryonic component Present Regionally restricted S1D Xist , S1D Merge Expression was detected in chorion.
Specimen S1E: embryonic day 7.0 (more )
Note: Double labeled: green - Xist; red - Atrx (assay MGI:6259659).
Structure Level Pattern Image Note
TS10: trophoblast giant cell Present S1E Merge , S1E Xist
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory