Probe:	Unc5c cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS23: cortical subplate	Strong		2e	Individual subplate cells with very strong labeling were seen across the entire rostrocaudal expanse of the cortex.

Structure	Level	Pattern	Image	Note
TS23: cortical subplate	Present		3g Wild-type	Sparse labeling was noted in the wild type.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	3g Reeler	Few, but clearly labeled cells were visible in the superplate of the reeler mutant.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Absent		4d E13.5

Structure	Level	Pattern	Image	Note
TS22: cortical subplate	Present		4d E14.5

Structure	Level	Pattern	Image	Note
TS23: cortical subplate	Present		4d E15.5
TS23: cortical plate	Present	Regionally restricted	4d E15.5	Expression was detected in the very lateral cortical plate.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Strong	Regionally restricted	4d E17.5	Expression was primarily in the lateral cortical plate.
TS25: cortical subplate	Weak		4d E17.5

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Weak		4d P8
TS28: cortical layer VI	Weak	Regionally restricted	4d P8	Expression was in upper layer VI.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Ubiquitous	4d Adult
TS28: cortical layer V	Weak		4d Adult
TS28: cortical layer VI	Weak	Regionally restricted	4d Adult	Expression was in upper layer VI.

Structure	Level	Pattern	Image	Note
TS23: cortical subplate	Present		5f , 5h , 5e	There was no colocalization with Gad1 labeling.

Structure	Level	Pattern	Image	Note
TS23: caudal ganglionic eminence	Present		S4k
TS23: cortical subplate	Present		S4k
TS23: thalamus	Present		S4k

Structure	Level	Pattern	Image	Note
TS28: basal ganglia	Present		S4k'
TS28: hippocampus	Present		S4k'
TS28: thalamus	Present		S4k'

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present
TS22: prethalamus	Present
TS22: thalamus	Present	Regionally restricted		Expression was detected in the medioventral part.

Structure	Level	Pattern	Image	Note
TS22: basal ganglia	Present	Regionally restricted		Expression was detected in the claustrum.
TS22: epithalamus	Present
TS22: prethalamus	Present
TS22: thalamus	Present	Regionally restricted		Expression was detected in the medioventral part.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Regionally restricted		Expression was detected in the claustrum.
TS23: dorsal lateral geniculate nucleus	Present
TS23: epithalamus	Present
TS23: habenula	Present
TS23: prethalamus	Present
TS23: thalamus	Present

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Regionally restricted		Expression was detected in the claustrum.
TS25: dorsal lateral geniculate nucleus	Present
TS25: main olfactory bulb	Present
TS25: thalamus	Present

Structure	Level	Pattern	Image	Note
TS28: claustrum	Ambiguous
TS28: hippocampus	Present	Single cells
TS28: thalamus	Present	Regionally restricted		Expression was especially noted in the ventromedial nucleus.

Structure	Level	Pattern	Image	Note
TS28: diencephalon	Weak	Ubiquitous
TS28: telencephalon	Weak	Ubiquitous