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Gene Expression Data
Assay Details
Assay
Reference: J:198109 Oeschger FM, et al., Gene expression analysis of the embryonic subplate. Cereb Cortex. 2012 Jun;22(6):1343-59
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6258913
Gene symbol: Gad1
Gene name: glutamate decarboxylase 1
Reporter: GFP
Antibody: anti-GFP (ab3080)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5a: embryonic day 15.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: green - Gad1; red - Cdh10 (assay MGI:6258664).
Structure Level Pattern Image Note
TS23: cerebral cortex marginal layer Present 5a
TS23: cortical subplate Present 5c , 5a , 5d There was no colocalization with Cdh10 staining.
TS23: cerebral cortex intermediate zone Present Regionally restricted 5a Expression was detected at the boundary between the intermediate zone and the subventricular zone.
TS23: cerebral cortex subventricular zone Present Regionally restricted 5a Expression was detected at the boundary between the intermediate zone and the subventricular zone.

Specimen 5e: embryonic day 15.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: green - Gad1; red - Unc5c (assay MGI:6258669).
Structure Level Pattern Image Note
TS23: cerebral cortex marginal layer Present 5e
TS23: cortical subplate Present 5h , 5e , 5g There was no colocalization with Unc5c staining.
TS23: cerebral cortex intermediate zone Present Regionally restricted 5e Expression was detected at the boundary between the intermediate zone and the subventricular zone.
TS23: cerebral cortex subventricular zone Present Regionally restricted 5e Expression was detected at the boundary between the intermediate zone and the subventricular zone.

Specimen 6A: embryonic day 15.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: green - Gad1; red - Rcan2 (assay MGI:6258918).
Structure Level Pattern Image Note
TS23: cerebral cortex marginal layer Present Regionally restricted 6Aa''' , 6Aa'' , 6Aa Expression was detected at the inner edge of the marginal layer. The Gad1-positive cells were a distinct population from the Rcan2-positive cells at the outer edge of the marginal zone.

Specimen 6D: postnatal day 8; Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: green - Gad1; red - Rcan2 (assay MGI:6258918).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 6Dd Coexpression was noted in most of the Rcan2-positive cells.
TS28: cortical layer V Present 6Dd Coexpression was noted in most of the Rcan2-positive cells.
TS28: cortical layer VI Present 6Dd Coexpression was noted in most of the Rcan2-positive cells.
TS28: cortical subplate Present 6Dd'' , 6Dd''' , 6Dd Coexpression was noted with only few Rcan2-positive cells.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory