Gene Expression Data
Assay Details
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Assay
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Reference:
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J:236243
Gittenberger-de Groot AC, et al., 14-3-3epsilon controls multiple developmental processes in the mouse heart. Dev Dyn. 2016 Nov;245(11):1107-1123
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6256741
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Gene symbol:
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Cdkn1b
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Gene name:
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cyclin dependent kinase inhibitor 1B
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Antibody:
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anti-p27 Kip1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3a:
embryonic day 13.5
(more  )
Specimen
3a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3b:
embryonic day 13.5
(more )
Specimen
3b:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3c:
embryonic day 13.5
(more )
Specimen
3c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: endocardial lining |
Present |
Regionally restricted |
3c
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Increased expression in endocardial cells with a cuboidal shape, variably seen, lining developing semilunar and atrioventricular valves.
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| TS21: outflow tract endocardial cushion |
Present |
Regionally restricted |
3c
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Expression in a few cells in endocardial cushion mesenchyme.
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Specimen
3d:
embryonic day 13.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
3d:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3e:
embryonic day 13.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
3e:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 13.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: semilunar valve |
Present |
Regionally restricted |
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Expression in leaflets of both mitral and tricuspid valves.
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| TS21: tricuspid valve |
Present |
Regionally restricted |
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Expression in leaflets of both mitral and tricuspid valves.
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Specimen
3f:
embryonic day 13.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
3f:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7c:
embryonic day 13.5
(more )
Specimen
7c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: visceral pericardium |
Weak |
Regionally restricted |
7c
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Authors report expression in fewer epicardial cells in the +/+ as compared to the -/- embryo.
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Specimen
7d:
embryonic day 13.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
7d:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: visceral pericardium |
Present |
Regionally restricted |
7d
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Authors report patchy up-regulation of expression in epicardium-derived cells within an area of subepicardial blebbing.
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Specimen
E14.5 +/+ not shown:
embryonic day 14.5
(more )
Specimen
E14.5 +/+ not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
E15.5 -/- not shown:
embryonic day 14.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
E15.5 -/- not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
E15.5 +/+ not shown:
embryonic day 15.5
(more )
Specimen
E15.5 +/+ not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
E15.5 -/- not shown:
embryonic day 15.5; Ywhaetm1Awb/Ywhae +
(more )
Specimen
E15.5 -/- not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ywhaetm1Awb/Ywhae+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools