Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44409
Gofflot F, et al., Genetic patterning of the developing mouse tail at the time of posterior neuropore closure. Dev Dyn. 1997 Dec;210(4):431-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6226142
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Wnt5a probe13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2G:
embryonic day 10.5
(more  )
Note: 28-30 somite stage.
Specimen
2G:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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28-30 somite stage.
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Specimen
3a:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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Specimen
3b:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: notochord |
Absent |
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3_Wnt5a b
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| TS17: tail |
Present |
Regionally restricted |
3_Wnt5a b
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All tissues adjacent to the tail bud, except the notochord, show expression signal.
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| TS17: tail surface ectoderm |
Present |
Regionally restricted |
3_Wnt5a b
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Surface ectoderm expression is confined to the ventral ectodermal ridge.
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| TS17: future spinal cord |
Very strong |
Regionally restricted |
3_Wnt5a b
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The open neural plate was very strong dorsolaterally.
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| TS17: tail mesenchyme |
Strong |
Regionally restricted |
3_Wnt5a b
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Beneath the neuropore, a high level of expression is observed in ventral and lateral regions; ventral cells located just above the ventral ectodermal ridge show the most intense labeling.
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Specimen
3c:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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Specimen
3d:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3d:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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Specimen
3e:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3e:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3_Wnt5a e
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Neuroepithelial expression was confined to the apical region and was progressively downregulated.
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