Gene Expression Data
Assay Details
Assay
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Reference:
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J:44409
Gofflot F, et al., Genetic patterning of the developing mouse tail at the time of posterior neuropore closure. Dev Dyn. 1997 Dec;210(4):431-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6226086
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Gene symbol:
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Rarg
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Gene name:
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retinoic acid receptor, gamma
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Probe:
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Rarg probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2F:
embryonic day 10.5
(more )
Note: 28-30 somite stage.
Specimen
2F:
(close )
Genetic Background:
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C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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28-30 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail |
Present |
Regionally restricted |
2F
|
Expression was detected at a low level in all tissues of the tail region, with a caudal border located just cranially to the most recently formed somite.
|
TS17: tail surface ectoderm |
Present |
|
2F
|
|
Specimen
3a:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail |
Present |
|
3_RAR gamma a
|
Central and dorsal cells show higher levels than the ventral/peripheral ones.
|
Specimen
3b:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail |
Present |
Non-Uniform |
3_RAR gamma b
|
Central and dorsal cells show higher levels than the ventral/peripheral ones.
|
TS17: future spinal cord |
Present |
Regionally restricted |
3_RAR gamma b
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High level of expression was detected in the neural plate of the open neuropore.
|
TS17: tail mesenchyme |
Present |
Non-Uniform |
3_RAR gamma b
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Within the mesoderm, the ventral midline area above the ventral ectodermal ridge was more strongly labelled than the lateral and paraxial regions.
|
TS17: tail surface ectoderm |
Weak |
Non-Uniform |
3_RAR gamma b
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Surface ectoderm was labeled at a low level except for the ventral ectodermal ridge, in which transcripts are undetectable.
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Specimen
3c:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3d:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3e:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
|