Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present	Regionally restricted	1A	Expression in region anterior to forelimb in restricted subpopulation of endothelial cells that will give rise to jugular lymph sacs.

Structure	Level	Pattern	Image	Note
TS17: anterior cardinal vein	Present	Regionally restricted	1B	Expression in endothelial cells - more obvious and abundant - beginning to bud from anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: anterior cardinal vein	Present	Regionally restricted	1C	Expression in increasing number of cells anterior to forelimb. Expression in endothelial cells beginning to bud from anterior cardinal vein.
TS19: cardiovascular system endothelium	Strong	Regionally restricted	1C	Budding and spreading of expressing cells from the anterior cardinal vein was clearly polarized.

Structure	Level	Pattern	Image	Note
TS20: anterior cardinal vein	Present	Regionally restricted	1D	Budding and spreading of expressing cells from the anterior cardinal vein was clearly polarized.
TS20: cardiovascular system endothelium	Present	Regionally restricted	1D	Expression in increased number of endothelial cells scattered along antero-posterior axis of embryo.

Structure	Level	Pattern	Image	Note
TS22: dermis	Present	Regionally restricted	1E	Expression in lymphatic vessels of dermis.
TS22: lymphatic system	Present	Regionally restricted	1E	Expression in jugular and perimesonephric regions. Expression in jugular and retroperitoneal lymph sacs.
TS22: liver	Strong		1E

Structure	Level	Pattern	Image	Note
TS23: dermis	Present	Regionally restricted	1F	Expression in jugular and perimesonephric regions. Expression in jugular and retroperitoneal lymph sacs.
TS23: lymphatic system	Present	Regionally restricted	1F	Expression in larger lymphatic vessels in deeper plexus.

Structure	Level	Pattern	Image	Note
TS16: anterior cardinal vein	Present	Regionally restricted	2A	Expression in a few endothelial cells lining wall of anterior cardinal vein - all located toward outer margin of vein.

Structure	Level	Pattern	Image	Note
TS19: cardiovascular system endothelium	Present	Regionally restricted	2C	Expression in endothelial cells moving dorsoanteriorly toward head region. Obvious regionalization of expressing cells in the cardinal vein. Budding and spreading of expressing cells from the anterior cardinal vein was clearly polarized. Also expression in cells near esophagus and lung buds.

Structure	Level	Pattern	Image	Note
TS22: cardiovascular system endothelium	Present	Regionally restricted	2D	Expression in endothelial cells surrounding lumen of lymphatic vessels and capillaries along the embryo body.
TS22: blood vessel endothelium	Absent		2D	No expression in endothelial cells of blood vessels containing erythrocytes.

Structure	Level	Pattern	Image	Note
TS23: lymphatic vessel	Present	Regionally restricted	2E	Expression in lymphatic capillary network of dermis, intermingled with blood vessels and blood capillaries.

Structure	Level	Pattern	Image	Note
TS19: anterior cardinal vein	Present	Regionally restricted	3B	Expression in cells in wall of cardinal vein and budding out from the vein.

Structure	Level	Pattern	Image	Note
TS22: dermis	Present	Regionally restricted	4B	Author report normal development of superficial lymphatic capillary network of dermis.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Absent		4C	Authors state no lymphatic vessels and capillaries were seen in homozygous littermates.

Structure	Level	Pattern	Image	Note
TS17: anterior cardinal vein	Present	Regionally restricted	5A	Authors state no obvious differences in the number of expressing cells in heterozygous and homozygous mutant littermates.

Structure	Level	Pattern	Image	Note
TS17: anterior cardinal vein	Present	Regionally restricted	5B	Authors state no obvious differences in the number of expressing cells in heterozygous and homozygous mutant littermates.

Structure	Level	Pattern	Image	Note
TS19: anterior cardinal vein	Present	Regionally restricted	5C	Expression in endothelial cells budding from the anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: anterior cardinal vein	Present	Regionally restricted	5D	Authors report a decrease in the number of endothelial cells budding from the anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS19: cardiovascular system endothelium	Present	Regionally restricted	5E	Expression in endothelial cells lining wall of cardinal vein and budding from the vein.

Structure	Level	Pattern	Image	Note
TS19: cardiovascular system endothelium	Present	Regionally restricted	5F	Authors report fewer endothelial cells lining wall of cardinal vein, and reduced budding from vein.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	5G	Authors report normal endothelial sprouting in dermis of Prox1+/- embryos.

Structure	Level	Pattern	Image	Note
TS21: lymphatic system	Absent		5H	No lymphatic vessels or capillaries were observed in same region of Prox1-/- embryos. Authors state lymph sacs, networks of lymphatic vessels, and superficial lymphatic capillaries were not developed in nullizygous embryos at this stage.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	7A	Expression in lymphatic cells around the esophagus, trachea and pulmonary bronchi.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Absent		7B	Authors report no expression in trachea, esophagus and lungs.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	7C +/-	Expression in lymphatic vessels in the lung.v
TS22: endocrine system	Present	Regionally restricted	7C +/-	Expression in neuroendocrine cells.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Absent		7C -/-	Authors report no expression in trachea, esophagus and lungs.
TS22: endocrine system	Absent		7C -/-	Expression in neuroendocrine cells.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	7D	Expression in lymphatic vessels in the mesenteries of intestines.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Absent		7E	No expression in the mesenteries of intestines.