Dnmt1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:135338 Cirio MC, et al., Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol. 2008;8:9
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6201325
Gene symbol:	Dnmt1
Gene name:	DNA methyltransferase 1

Antibody:	UPT82
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Antibody recognizes only the Dnmt1s proteins.

Results

3B upper +/+ left ©

Specimen 3B +/+ MII: postnatal day 21-35 (more

)

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		3B upper +/+ left

3B upper +/+ middle ©

Specimen 3B +/+ PN3: embryonic day 0.5 (more

)
Note: 1-cell embryo, pronuclear stage 3

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		3B upper +/+ middle	Only expressed in the cytoplasm, not in the pronuclei.

3B upper +/+ right ©

Specimen 3B +/+ PN4: embryonic day 0.5 (more

)
Note: 1-cell embryo, pronuclear stage 4

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		3B upper +/+ right	Only expressed in the cytoplasm, not in the pronuclei.

3B upper v/v left ©

Specimen 3B v/v MII: postnatal day 21-35; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Absent		3B upper v/v left

3B upper v/v middle ©

Specimen 3B v/v PN3: embryonic day 0.5; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 1-cell embryo, pronuclear stage 3

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Absent		3B upper v/v middle

3B upper v/v right ©

Specimen 3B v/v PN4: embryonic day 0.5; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 1-cell embryo, pronuclear stage 4

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Absent		3B upper v/v right

3B lower 1s/1o/1s/1o left ©

Specimen 3B 1s/1o PN3: embryonic day 0.5; Dnmt1^tm1Jamt/Dnmt1^tm1Jamt (more

)
Note: 1-cell embryo, pronuclear stage 3

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Strong		3B lower 1s/1o/1s/1o left	Increased expression. Cytoplasmic staining was significantly higher than nuclear staining.

3B lower 1s/1o/1s/1o right ©

Specimen 3B 1s/1o PN4: embryonic day 0.5; Dnmt1^tm1Jamt/Dnmt1^tm1Jamt (more

)
Note: 1-cell embryo, pronuclear stage 4

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Strong		3B lower 1s/1o/1s/1o right	Increased expression. Cytoplasmic staining was significantly higher than nuclear staining.

4A 2-cell ©

Specimen 4A +/+ 2-cell: embryonic day 1.5 (more

)
Note: 2-cell embryo

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		4A 2-cell	Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4A 4-cell ©

Specimen 4A +/+ 4-cell: embryonic day 2.0 (more

)
Note: 4-cell embryo

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		4A 4-cell	Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4A 8-cell ©

Specimen 4A +/+ 8-cell: embryonic day 2.5 (more

)
Note: 8-cell embryo

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		4A 8-cell	Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4A Mo ©

Specimen 4A Mo: embryonic day 3.0 (more

)
Note: Morula

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		4A Mo	Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4A Bl ©

Specimen 4A Bl: embryonic day 3.5 (more

)
Note: Blastocyst

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		4A Bl	Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4B 1-cell ©

Specimen 4B 1-cell: embryonic day 0.5; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 1-cell embryo from female V/V with male +/+ cross.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Absent		4B 1-cell	Dnmt1s was synthesized zygotically from the paternal allele.

4B 2-cell ©

Specimen 4B 2-cell: embryonic day 1.5; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 2-cell embryo from female V/V with male +/+ cross.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		4B 2-cell	Dnmt1s was synthesized zygotically from the paternal allele. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4B 4-cell ©

Specimen 4B 4-cell: embryonic day 2.0; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 4-cell embryo from female V/V with male +/+ cross.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		4B 4-cell	Dnmt1s was synthesized zygotically from the paternal allele. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

4B 8-cell ©

Specimen 4B 8-cell: embryonic day 2.5; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 8-cell embryo from female V/V with male +/+ cross.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		4B 8-cell	Dnmt1s was synthesized zygotically from the paternal allele. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

Specimen 4C upper 2-cell: embryonic day 1.5; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 2-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		4C upper 2-cell	Expressed both maternally and zygotically. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

Specimen 4C upper 4-cell: embryonic day 2.0; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 4-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		4C upper 4-cell	Expressed both maternally and zygotically. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

Specimen 4C upper 8-cell: embryonic day 2.5; Dnmt1^tm2Jrc/Dnmt1⁺ (more

)
Note: 8-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		4C upper 8-cell	Expressed both maternally and zygotically. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

Specimen 4C lower 2-cell: embryonic day 1.5; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 2-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		4C lower 2-cell	Expressed maternally. Expressed in the cytoplasm and the nuclei, more concentrated in the nuclei.

Specimen 4C lower 4-cell: embryonic day 2.0; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 4-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Absent		4C lower 4-cell

Specimen 4C lower 8-cell: embryonic day 2.5; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 8-cell embryo from female V/+ with male V/V cross.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent		4C lower 8-cell

Specimen not shown: embryonic day 0.5 (more

)
Note: 1-cell embryo, pronuclear stage 1

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present			Only expressed in the cytoplasm, not in the pronuclei.

Specimen not shown: embryonic day 0.5 (more

)
Note: 1-cell embryo, pronuclear stage 2

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present			Only expressed in the cytoplasm, not in the pronuclei.

Specimen not shown: embryonic day 0.5; Dnmt1^tm2Jrc/Dnmt1^tm2Jrc (more

)
Note: 1-cell embryo, pronuclear stage 1 from female V/V with male V/V cross.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Absent

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