Antibody:	anti-Stat3 (C-20) sc-482
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Stat3 (C-20-G) antibody MGI:6201084 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	4A	Authors report cytoplasmic expression closely paralleling the polarized expression pattern seen for leptin. Nuclear expression observed in GV oocytes.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	4B	Authors report cytoplasmic expression closely paralleling the polarized expression pattern seen for leptin. Nuclear expression observed in GV oocytes.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	4C	Authors report cytoplasmic expression closely paralleling the polarized expression pattern seen for leptin. Nuclear expression observed in both pronuclei.
TS1: second polar body	Present		4C

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	4D	Authors report cytoplasmic expression closely paralleling the polarized expression pattern seen for leptin. Nuclear expression observed in both pronuclei.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	4E	Each blastomere characterized by intense expression in subplasmalemmal cytoplasm, diminishing progressively in subjacent cortical cytoplasm.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Weak	Regionally restricted	4F	Authors report low intensity nuclear expression at 2-cell stage. Each blastomere characterized by intense expression in subplasmalemmal cytoplasm, diminishing progressively in subjacent cortical cytoplasm. No nucleolar expression.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present	Regionally restricted	4G	Authors report the relative intensitiy of expression was blastomere specified. Little expression in at least one blastomere.
TS3: second polar body	Present		4G

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present	Regionally restricted	4H	Authors report the relative intensitiy of expression was blastomere specified. Little expression in at least one blastomere.
TS3: second polar body	Present		4H

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	4I	Authors report virtually no expression in two of 8 blastomeres.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	4J	Authors report virtually no expression in two of 8 blastomeres. Typically, the expression-deficient cells (blastomere 6 and 8) were paired and located on one side of the embryo.
TS3: second polar body	Present		4J

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Weak	Regionally restricted	4K	Substantially reduced expression in a region of outer cells.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present	Regionally restricted	4L	Authors report pattern of unequal distribution evident in individual optical sections. Expression not seen in all outer cells.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	5A	No expression in inner cell mass and portion of adjacent trophoblast. Authors report expression followed the same pattern as observed for leptin.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Absent		5B	Expression undetectable or at background levels in inner cell mass.
TS4: trophectoderm	Present	Regionally restricted	5B	Expression localized to apical surface of individual trophoblast cells. Authors report expression followed the same pattern as observed for leptin.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	5C	Authors report differential expression in inner cell mass and trophoblast. Authors report expression followed the same pattern as observed for leptin.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Absent		5D	Expression undetectable or at background levels in inner cell mass.
TS4: trophectoderm	Present	Regionally restricted	5D	Expression localized to apical surface of individual trophoblast cells. Authors report expression for followed the same pattern as observed leptin.

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Present	Regionally restricted	5E	Expression in coronal cell process (that traverse the zona pellucida. Relatively intense expression in bulbous structures at distal end of transzonal processes that appeared to invaginate the oolemma.

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Present	Regionally restricted	5F	Expression in apparent external aspect of invaginations that resembled endocytotic pits.
TS28: oocyte	Strong	Regionally restricted	5F	Intense expression in subjacent cytoplasm of oocyte.

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Present	Regionally restricted	5G	Expression in apparent external aspect of invaginations that resembled endocytotic pits.
TS28: oocyte	Strong	Regionally restricted	5G	Intense expression in subjacent cytoplasm of oocyte.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	5I	Polarized domain of expression in granulosa cells and oocyte.
TS28: ovary follicle	Present	Regionally restricted	5I	Expression along outer perimeter of cumulus oophorus. Expression in both cumulus oophorus and corona radata.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	5J	Polarized domain of expression in oocyte. Authors state the pattern of expression seen with C57BL/6J/ob/ob was virtually identical to that of normal mice.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted		Authors state the pattern of expression seen with C57BL/Ks db/db was virtually identical to that of normal mice.