Antibody:

OB (Y-20) antibody

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	1A	Authors report expression was localized asymmetrically. Distinct domain of intense expression.
TS28: ovary follicle	Present	Regionally restricted	1A	Authors report most intense expression in region of cumulus and coronal cells associated wtih oolemma and subjacent ooplasm with most intense expression. Intense expression extended through tranzonal processes to surface of oocyte.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	1B	Most expression concentrated in one portion of oocyte and extended from plasma membrane into subplasmalemmal cytoplasm. Intense expression extended through tranzonal processes to surface of oocyte.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	1D	Authors report expression was localized asymmetrically. Distinct domain of intense expression.
TS1: second polar body	Present		1D

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		1E	Most expression concentrated in one portion of oocyte and extended from plasma membrane into subplasmalemmal cytoplasm.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		1G

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		1H

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage conceptus	Present	Regionally restricted	2A	Authors state first cleavage furrow develops in expression domain containing second polar body.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Ambiguous	Regionally restricted	2B	Authors report absence of expression in an internal section.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	2C	Authors state first cleavage furrow develops in expression domain containing second polar body. Each blastomere characterized by intense expression in subplasmalemmal cytoplasm, diminishing progressively in subjacent cortical cytoplasm.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	2D	Authors report high level of expression in blastomere 1; reduced expression in blastomeres 2 and 3.
TS2: second polar body	Present		2D

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2E

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2F

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present	Regionally restricted	2G	Expression very hign in blastomere 3, substantially reduced in a blastomere 4, and of intermediate and variable intensity in the remaining blastomeres 1 and 2.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	2H	Authors report virtually no expression in two of 8 blastomeres. Typically, the expression-deficient cells (blastomere 2 and 3) were paired and located on one side of the embryo.
TS3: second polar body	Absent		2H	Authors state the second polar body was located in the leptin/Stat3-poor domain

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Weak	Regionally restricted	2I	Reduced expression in inner cells.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Trace	Regionally restricted	2J	Cytoplasmic expression was undetectable or marginally above background in inside cells of morula.
TS4: mural trophectoderm	Strong		2J	Expression most intense in mural trophoblast.
TS4: polar trophectoderm	Weak		2J	Expression progressively diminished towards polar trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	2K	Authors report pattern of unequal distribution evident in individual optical sections. In some sections, expression in all outer cells.

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Present	Regionally restricted	5H	Intense expression in zona pellucida-associated coronal cell processes.
TS28: oocyte	Present	Regionally restricted	5H	Authors report polarized pattern expression virtually identical to oocytes of normal animals (e.g. Figure 1A).

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted		Authors state germinal vesicle oocytes from C57BL/Ks db/db mice exhibited a polarized pattern expression virtually identical to oocytes of normal animals (e.g. Figure 1A).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6D	Authors report differential expression in inner cell mass and trophoblast.
TS4: trophectoderm	Present	Regionally restricted	6D	Reduced expression in trophoblast. Expression localized to apical surface of individual trophoblast cells.
TS4: mural trophectoderm	Strong		6D	Expression most intense in mural trophoblast.
TS4: polar trophectoderm	Weak		6D	Expression progressively diminished towards polar trophoblast.
TS4: inner cell mass	Absent		6D

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Absent		6E upper
TS4: trophectoderm	Present	Regionally restricted	6E upper	Reduced expression in trophoblast. Expression localized to apical surface of individual trophoblast cells.
TS4: mural trophectoderm	Strong		6E upper	Expression most intense in mural trophoblast.
TS4: polar trophectoderm	Weak		6E upper	Expression progressively diminished towards polar trophoblast.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Absent		6E lower
TS4: trophectoderm	Present	Regionally restricted	6E lower	Reduced expression in trophoblast. Expression localized to apical surface of individual trophoblast cells.
TS4: mural trophectoderm	Strong		6E lower	Expression most intense in mural trophoblast.
TS4: polar trophectoderm	Weak		6E lower	Expression progressively diminished towards polar trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6F	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6G	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6H	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6I	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6J	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Regionally restricted	6K	Authors report differential expression in inner cell mass and trophoblast.

Structure	Level	Pattern	Image	Note
TS28: ovary growing follicle	Present	Regionally restricted	7A	Expression within mass of follicle cells surrounding germinal vesicle stage oocytes. Expression completely around oocyte at interface with follicle cells. Intense expression in a subset of cells localized to one region of corona radiata and some overlying granulosa cells.

Structure	Level	Pattern	Image	Note
TS28: ovary growing follicle	Present	Regionally restricted	7B	Polarized domain of expression extending across all follicle cell layers to basement membrane. Authors report the same asymmetric localization of expression as in earlier growing follicles. Domain of intesnse expression extending from coronal cells associated with zona pellucida, through overying layers of granulosa cells, up to inner surface of basement membrane.

Structure	Level	Pattern	Image	Note
TS28: ovary growing follicle	Present	Regionally restricted	7C	Expression at interface between coronal cells and oocyte. Expression concentrated near surface of oolemma in expressing region of oocyte.
TS28: oocyte	Present	Regionally restricted	7C	Expression in corresponding portion of oocyte.

Structure	Level	Pattern	Image	Note
TS28: ovary growing follicle	Present	Regionally restricted	7D	Expression in coronal cell processes within the zona pellucida. Intense expression in coronal cells, extending through tranzonal processes to surface of oocyte.
TS28: oocyte	Present	Regionally restricted	7D	Expression in corresponding portion of oocyte.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		7K