Antibody:	Anti-Robo1 (AF1749)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Rabbit anti-Robo1 MGI:6188838 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Widespread	1_Robo1 E13.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS21: medial ganglionic eminence	Strong	Regionally restricted	1_Robo1 E13.5 Middle	Strong expression throughout mantle zone of medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Widespread	1_Robo1 E13.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo1 E15.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo1 E15.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo1 E15.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo1 E15.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo1 E15.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo1 E15.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong	Regionally restricted		Strong expression in preplate layer.
TS23: cerebral cortex mantle layer	Strong
TS23: cortical subplate	Strong
TS23: cerebral cortex intermediate zone	Strong	Regionally restricted		Strong expression in tangential migratory routes of interneurons, especially within intermediate zone/subventricular zone.
TS23: cerebral cortex subventricular zone	Strong	Regionally restricted		Strong expression in tangential migratory routes of interneurons, especially within intermediate zone/subventricular zone.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo1 E17.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo1 E17.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo1 E17.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo1 E17.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo1 E17.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo1 E17.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted		Expression somewhat downregulated in ventral telencephalon.
TS25: cerebral cortex subventricular zone	Strong

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		2A	Expression in interneurons throughout the cortical anlage.
TS23: cerebral cortex intermediate zone	Present	Regionally restricted	2A	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cerebral cortex subventricular zone	Present	Regionally restricted	2A	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cortical subplate	Present	Regionally restricted	2A	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	2B	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cortical subplate	Present	Regionally restricted	2B	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	2C	Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		S1J +/+

Structure	Level	Pattern	Image	Note
TS20: brain	Absent		S1J -/-