Mllt6 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:169020 Zhang Z, et al., Widely expressed Af17 is likely not required for embryogenesis, hematopoiesis, and animal survival. Genesis. 2010 Dec;48(12):693-706
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6161206
Gene symbol:	Mllt6
Gene name:	myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6

Reporter:	lacZ
	Direct Detection

Results

3a ©

Specimen 3a: embryonic day 10.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		3a
TS17: olfactory placode	Present	Regionally restricted	3a	Expressed in the margin of the olfactory pit.
TS17: forebrain	Present	Regionally restricted	3a	Expressed in the rostral end of the forebrain around the dorsal midline.
TS17: forelimb bud	Present	Regionally restricted	3a	Relatively weak staining at the edge of the forelimb bud.

3b ©

Specimen 3b: embryonic day 10.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		3b
TS17: olfactory placode	Present	Regionally restricted	3b	Expressed in the margin of the olfactory pit.
TS17: forebrain	Present	Regionally restricted	3b	Expressed in the rostral end of the forebrain around the dorsal midline.
TS17: forelimb bud	Present	Regionally restricted	3b	Relatively weak staining at the edge.
TS17: tail	Present		3b	Relatively weak staining at the tail bud.

3d ©

Specimen 3d: embryonic day 10.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3d	Expressed in the neuroepithelium, but not adjacent mesoderm or ectoderm.

4a ©

Specimen 4a: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present		4a
TS20: central nervous system	Present	Regionally restricted	4a	Expressed in the midline.
TS20: dorsal root ganglion	Present		4a

4b ©

Specimen 4b: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain roof plate	Present	Regionally restricted	4b	Expressed along the middle.
TS20: central nervous system	Present	Regionally restricted	4b	Expressed in the midline.
TS20: dorsal root ganglion	Present		4b

4c ©

Specimen 4c: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4c	Expressed in the median walls of telencephalic vesicles.
TS20: nasal epithelium	Present		4c

4d ©

Specimen 4d: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: eye	Present		4d

4f ©

Specimen 4f: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4f	Expressed in the median wall of the telencephalic vesicles.

4g ©

Specimen 4g: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: eye	Present		4g	With the basal (lens) side of the neural retina being stained to a greater extent.

4h ©

Specimen 4h: embryonic day 12.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Image
TS20: heart	Strong	4h
TS20: metanephros	Strong	4h
TS20: mesonephric tubule	Present	4h

4i ©

Specimen 4i: embryonic day 14.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		4i

4k ©

4l ©

Specimen 4k/l: embryonic day 14.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		4k , 4l

5Brain +/- ©

Specimen 5 Brain +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: brain	Present		5Brain +/-	Weaker expression compared to homozygous mutant.

5Brain -/- ©

Specimen 5 Brain -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: brain	Present		5Brain -/-

5Kidney +/- ©

Specimen 5 Kidney +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)
Note: Wild-type had some noticeable background X-gal staining.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5Kidney +/-	Weaker expression compared to homozygous mutant.

5Kidney -/- ©

Specimen 5 Kidney -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)
Note: Wild-type had some noticeable background X-gal staining.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5Kidney -/-

5Testis +/- ©

Specimen 5 Testis +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: testis	Present		5Testis +/-	Weaker expression compared to homozygous mutant.

5Testis -/- ©

Specimen 5 Testis -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: testis	Present		5Testis -/-

5Eye +/- ©

Specimen 5 Eye +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: eye	Present		5Eye +/-	Weaker expression compared to homozygous mutant.

5Eye -/- ©

Specimen 5 Eye -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: eye	Present		5Eye -/-

5Bladder +/- ©

Specimen 5 Bladder +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: bladder	Present		5Bladder +/-	Weaker expression compared to homozygous mutant.

5Bladder -/- ©

Specimen 5 Bladder -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: bladder	Present		5Bladder -/-	Weaker expression.

5Heart +/- ©

Specimen 5 Heart +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: heart	Present		5Heart +/-	Weaker expression compared to homozygous mutant.

5Heart -/- ©

Specimen 5 Heart -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: heart	Present		5Heart -/-

5Lung +/- ©

Specimen 5 Lung +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: lung	Present		5Lung +/-	Weaker expression compared to homozygous mutant.

5Lung -/- ©

Specimen 5 Lung -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: lung	Present		5Lung -/-

5Spleen +/- ©

Specimen 5 Spleen +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: liver	Absent		5Spleen +/-

5Spleen -/- ©

Specimen 5 Spleen -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: spleen	Absent		5Spleen -/-

5Liver +/- ©

Specimen 5 Liver +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: liver	Absent		5Liver +/-

5Liver -/- ©

Specimen 5 Liver -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: liver	Absent		5Liver -/-

5Stomach +/- ©

Specimen 5 Stomach +/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: stomach	Present		5Stomach +/-	Weaker expression compared to homozygous mutant.

5Stomach -/- ©

Specimen 5 Stomach -/-: embryonic day 16.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS24: stomach	Present		5Stomach -/-	Weaker expression.

6a Brain -/- ©

Specimen 6a Brain: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: brain	Present		6a Brain -/-

6a Kidney -/- ©

Specimen 6a Kidney: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present		6a Kidney -/-

6a Testis -/- ©

Specimen 6a Testis: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		6a Testis -/-

6a Eye -/- ©

Specimen 6a Eye: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: eye	Present		6a Eye -/-

6a Bladder -/- ©

Specimen 6a Bladder: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: bladder	Present		6a Bladder -/-

6a Heart -/- ©

Specimen 6a Heart: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: heart	Present		6a Heart -/-

6a Lung -/- ©

Specimen 6a Lung: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: lung	Present		6a Lung -/-

6a Spleen -/- ©

Specimen 6a Spleen: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen	Present		6a Spleen -/-

6a Penis -/- ©

Specimen 6a Penis: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: penis	Absent		6a Penis -/-

6a Liver -/- ©

Specimen 6a Liver: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Absent		6a Liver -/-

Specimen 6a Small intestine: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: small intestine	Absent		6a Small intestine -/-

Specimen 6a Stomach: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: stomach	Present		6a Stomach -/-

Specimen 6a Salivary Gland: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: salivary gland	Present		6a Salivary Gland -/-

Specimen 6a Uterus: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: uterus	Present		6a Uterus -/-

7a ©

7b ©

7b' ©

Specimen 7a/b/b': postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7b' , 7b , 7a
TS28: hippocampus	Present		7b' , 7b , 7a
TS28: deep cerebellar nucleus	Present	Regionally restricted	7a , 7b' , 7b	Expressed in some deep nuclei.
TS28: hippocampus region	Strong		7b
TS28: dentate gyrus	Ambiguous		7b' , 7a	Almost complete absence of staining.

7c ©

7d ©

Specimen 7c/d: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		7c , 7d
TS28: cerebellum granule cell layer	Absent		7c , 7d

7e ©

7f ©

Specimen 7e/f: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)
Note: Double labeled: blue - Mllt6; brown - Gfap (assay MGI:6161210).

Structure	Level	Pattern	Image	Note
TS28: brain	Present		7e , 7f	Not colabeled by anti-GFAP antibody.

7g ©

7h ©

Specimen 7g/h: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)
Note: Double labeled: blue - Mllt6; brown - Rbfox3 (assay MGI:6161211).

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Present		7g , 7h	Largely, but not completely overlap with the staining of Rbfox3.

7i ©

7j ©

Specimen 7i/j: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)
Note: Double labeled: blue - Mllt6; brown - Rbfox3 (assay MGI:6161211).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7j , 7i	Largely, but not completely overlap with the staining of Rbfox3.

8a ©

8b ©

8c ©

Specimen 8a/b/c: embryonic day 17.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Strong		8b , 8a , 8c
TS25: spinal cord	Present		8b , 8a , 8c

8f ©

Specimen 8f: embryonic day 17.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS25: renal cortex	Present	Scattered	8f	Sparsely expressed.
TS25: renal medulla	Present	Scattered	8f	Sparsely expressed.

Specimen not shown: postnatal week 6-8; Mllt6^{Gt(AC0433)Wtsi}/Mllt6^{Gt(AC0433)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Absent

Specimen not shown: embryonic day 10.5; Mllt6^{Gt(AC0433)Wtsi}/Mllt6⁺ (more

)

Structure	Level	Note
TS17: forebrain	Present	Same pattern as in homozygous, just weaker.
TS17: forelimb bud	Present	Same pattern as in homozygous, just weaker.
TS17: midbrain-hindbrain junction	Present	Same pattern as in homozygous, just weaker.
TS17: olfactory placode	Present	Same pattern as in homozygous, just weaker.
TS17: tail	Present	Same pattern as in homozygous, just weaker.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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