Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:119866
Bort R, et al., Hex homeobox gene controls the transition of the endoderm to a pseudostratified, cell emergent epithelium for liver bud development. Dev Biol. 2006 Feb 1;290(1):44-56
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:6157925
|
|
Gene symbol:
|
Hhex
|
|
Gene name:
|
hematopoietically expressed homeobox
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1E:
embryonic day 8.5; Hhextm1Rbe/Hhex +
(more  )
Note: 10 somites (stage I). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
Specimen
1E:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
10 somites (stage I). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
|
Specimen
1F:
embryonic day 9.5; Hhextm1Rbe/Hhex +
(more )
Note: 21 somites (stage II). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
Specimen
1F:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
21 somites (stage II). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
|
Specimen
1G:
embryonic day 9.5; Hhextm1Rbe/Hhex +
(more )
Note: 27 somites (stage III). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
Specimen
1G:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
27 somites (stage III). Triple labeled: blue - Hhex reporter; red - Foxa2 (assay MGI:6157937); green - DAPI (false-colored).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: hepatic primordium |
Present |
Regionally restricted |
1G
|
Expression in emerging hepatic cells.
|
Specimen
3E:
embryonic day 9.0; Hhextm1Rbe/Hhex +
(more )
Note: 13 somites (early stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
13 somites (early stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
|
Specimen
3F:
embryonic day 9.0; Hhextm1Rbe/Hhex +
(more )
Note: ~20 somites (late stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
~20 somites (late stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
|
Specimen
3G:
embryonic day 9.0; Hhextm1Rbe/ Hhextm1Rbe
(more )
Note: 13 somites (early stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
Specimen
3G:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhextm1Rbe
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
13 somites (early stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
|
Specimen
3H:
embryonic day 9.0; Hhextm1Rbe/ Hhextm1Rbe
(more )
Note: ~20 somites (late stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhextm1Rbe
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
~20 somites (late stage II). Triple labeled: blue - Hhex reporter; red - phosphohistone H3; green - DAPI (false-colored).
|
Specimen
3M:
embryonic day 9.0; Hhextm1Rbe/Hhex +
(more )
Note: 12-16 somites (early stage II)
Specimen
3M:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12-16 somites (early stage II)
|
Specimen
3N:
embryonic day 9.0; Hhextm1Rbe/Hhex +
(more )
Note: 12-16 somites (early stage II)
Specimen
3N:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhex+
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12-16 somites (early stage II)
|
Specimen
3O:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhextm1Rbe
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12-16 somites (early stage II)
|
Specimen
3P:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhextm1Rbe
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12-16 somites (early stage II)
|
Specimen
6I:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Hhextm1Rbe/Hhextm1Rbe
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: gut endoderm |
Present |
Regionally restricted |
6I
|
Expression in what appear to be like duodenal cells.
|
|
Analysis Tools