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Gene Expression Data
Assay Details
Assay
Reference: J:236149 Edwards W, et al., Identification of a novel progenitor cell marker, grainyhead-like 2 in the developing pituitary. Dev Dyn. 2016 Nov;245(11):1097-1106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6156396
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: anti-PCNA
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
Assay notes: A Zinc fixative was used.
Results
Specimen 7A: postnatal day 1 (more )
Note: Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7B , 7A Expression is dorsal and in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7A , 7B Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7C: postnatal day 1 (more )
Note: Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Present Regionally restricted 7D , 7C Expression is dorsal and in the cleft cells.
Specimen 7E: postnatal day 1 (more )
Note: Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7F , 7E Expression is in the cleft cells and scattered in the parenchyma.
Specimen 7G: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Single cells 7G , 7H Expression is in the cleft cells.
TS27: adenohypophysis Present Regionally restricted 7H , 7G Expression in the parenchyma and cleft cells is the same as in wild-type.
Specimen 7I: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis pars intermedia Weak Single cells 7I , 7J Expression is in the cleft cells.
Specimen 7K: postnatal day 1; Foxg1tm1(cre)Skm/Foxg1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Double labeled: green - Pcna; red - Grhl2 (assay MGI:6155596).
Structure Level Pattern Image Note
TS27: adenohypophysis Present Regionally restricted 7L , 7K Expression in the parenchyma and cleft cells is the same as in wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory