Ccdc88c Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:251823 Takagishi M, et al., Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus. Cell Rep. 2017 Jul 25;20(4):960-972
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6155670
Gene symbol:	Ccdc88c
Gene name:	coiled-coil domain containing 88C

Antibody:	anti-Daple
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

4A +/+ ©

Specimen 4A +/+: postnatal day 21 (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	4A +/+	Authors state that fixation with methanol only results in expression in anterior cell membrane at the apical surface.

4A -/- ©

Specimen 4A -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Absent		4A -/-	No expression in ependymal cells.

4B ©

4B (inset) ©

4B Daple ©

Specimen 4B: postnatal day 21 (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin. Specimen sequentially fixed with methanol and paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	4B , 4B (inset) , 4B Daple	Authors report sequential fixation with methanol and paraformaldehyde resulted in expression near basal bodies.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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