Ctnnb1 Immunohistochemistry Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:251823 Takagishi M, et al., Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus. Cell Rep. 2017 Jul 25;20(4):960-972
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6155669
Gene symbol:	Ctnnb1
Gene name:	catenin beta 1

Antibody:	anti-beta-catenin (#610153), clone 14
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

3A Anterior dorsal +/+ ©

Specimen 3A Anterior dorsal +/+: postnatal day 21 (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Anterior dorsal +/+	Expression in intracellular junctions.

3A Anterior dorsal -/- ©

Specimen 3A Anterior dorsal -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Anterior dorsal -/-	Expression in intracellular junctions.

3A Anterior ventral +/+ ©

Specimen 3A Anterior ventral +/+: postnatal day 21 (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Anterior ventral +/+	Expression in intracellular junctions.

3A Anterior ventral -/- ©

Specimen 3A Anterior ventral -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Anterior ventral -/-	Expression in intracellular junctions.

3A Posterior +/+ ©

Specimen 3A Posterior +/+: postnatal day 21 (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Posterior +/+	Expression in intracellular junctions.

3A Posterior -/- ©

Specimen 3A Posterior -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	3A Posterior -/-

3D +/+ ©

Specimen 3D +/+: postnatal day 2 (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS27: lateral ventricle ependyma	Present	Regionally restricted	3D +/+	Expression in cell membranes.

3D -/- ©

Specimen 3D -/-: postnatal day 2; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Double labeled: red - Ctnnb1; green - gamma-tubulin.

Structure	Level	Pattern	Image	Note
TS27: lateral ventricle ependyma	Present	Regionally restricted	3D -/-	Expression in cell membranes.

4A +/+ ©

Specimen 4A +/+: postnatal day 21 (more

)
Note: Triple labeled: red - Ctnnb1; green - Ccdc88c (assay MGI:6155670); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		4A +/+

4A -/- ©

Specimen 4A -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Triple labeled: red - Ctnnb1; green - Ccdc88c (assay MGI:6155670); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		4A -/-

4B ©

Specimen 4B: postnatal day 21 (more

)
Note: Triple labeled: red - Ctnnb1; green - Ccdc88c (assay MGI:6155670); purple - gamma tubulin. Specimen sequentially fixed with methanol and paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		4B

4E merge +/+ ©

Specimen 4E +/+: postnatal day 21 (more

)
Note: Triple labeled: red - Ctnnb1; green - Dvl2 (assay MGI:6155673); blue - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		4E merge +/+

4E merge -/- ©

Specimen 4E -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Triple labeled: red - Ctnnb1; green - Dvl2 (assay MGI:6155673); blue - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		4E merge -/-

5D upper right ©

Specimen 5D +/+: postnatal day 9 (more

)
Note: Triple labeled: red - Ctnnb1; green - Fzd6 (assay MGI:6155674); blue - gamma tubulin. Specimen fixed with 90% methanol and 10% formalin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		5D upper right

5D lower right ©

Specimen 5D -/-: postnatal day 9; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Triple labeled: red - Ctnnb1; green - Fzd6 (assay MGI:6155674); blue - gamma tubulin. Specimen fixed with 90% methanol and 10% formalin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present		5D lower right

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