Probe:	Hoxd11 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present		3C
TS21: sclerotome	Present		3C

Structure	Level	Pattern	Image	Note
TS21: sclerotome	Present		3D	Expression extends anteriorly in the sclerotome of the mutant.
TS21: central nervous system	Present		3D	Expression was not extended anteriorly in the mutant central nervous system.

Structure	Level	Pattern	Image	Note
TS18: limb	Present

Structure	Level	Pattern	Image	Note
TS18: limb	Weak			Staining intensity was reduced in mutant limb, but there was no qualitative change in the distribution of the transcript domain.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		4E
TS19: sclerotome	Present		4E
TS19: forelimb bud	Present		4E	A proximal-posterior domain of limb expression was noted.
TS19: hindlimb bud	Present		4E	A proximal-posterior domain of limb expression was noted.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	4F	No changes in the anterior expression limit were noted in the mutant.
TS19: sclerotome	Present	Regionally restricted	4F	No changes in the anterior expression limit were noted in the mutant.
TS19: forelimb bud	Present	Regionally restricted	4F	No changes in expression pattern were noted in the mutant, including the proximal-posterior domain of limb expression.
TS19: hindlimb bud	Present	Regionally restricted	4F	No changes in expression pattern were noted in the mutant, including the proximal-posterior domain of limb expression.

Structure	Level	Pattern	Image	Note
TS15: tail	Present

Structure	Level	Pattern	Image	Note
TS15: tail	Present			No changes in expression pattern were noted in the mutant.

Structure	Level	Pattern	Image	Note
TS20: limb	Present

Structure	Level	Pattern	Image	Note
TS20: limb	Present			No changes in expression pattern were noted in the mutant.