Bmp7 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39018 Dudley AT, et al., Overlapping expression domains of bone morphogenetic protein family members potentially account for limited tissue defects in BMP7 deficient embryos. Dev Dyn. 1997 Mar;208(3):349-62
Assay type:	RNA in situ
MGI Accession ID:	MGI:6154056
Gene symbol:	Bmp7
Gene name:	bone morphogenetic protein 7

Probe:	Bmp7 cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

1A ©

Specimen 1A: embryonic day 8.5 (more

)

Structure	Level	Image
TS13: gut	Strong	1A
TS13: notochord	Strong	1A
TS13: surface ectoderm	Strong	1A
TS13: yolk sac endoderm	Strong	1A
TS13: optic pit neural ectoderm	Present	1A
TS13: future prosencephalon	Absent	1A
TS13: neural tube	Absent	1A

2A ©

Specimen 2A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Strong		2A
TS13: primitive ventricle cardiac muscle	Strong		2A

3A ©

Specimen 3A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Strong	Regionally restricted	3A	Expression is in the future pigmented epithelium and in the anterior region.
TS15: eye surface ectoderm	Strong		3A
TS15: lens placode	Strong		3A
TS15: optic stalk	Strong		3A
TS15: optic cup	Weak	Regionally restricted	3A	Expression is in the future neural retina.

3C ©

Specimen 3C: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: eye surface ectoderm	Present		3C
TS15: lens placode	Present		3C

4A ©

Specimen 4A: embryonic day 11.0-11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Present		4A
TS19: pigmented retinal epithelium	Present		4A
TS19: surface ectoderm	Present		4A
TS19: head mesenchyme	Present	Regionally restricted	4A	Expression is in the mesenchyme surrounding the eye.
TS19: cornea	Weak		4A
TS19: lens vesicle	Absent		4A
TS19: neural retina	Absent		4A

5A ©

Specimen 5A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Strong		5A
TS19: mesenchyme of metanephros	Strong	Regionally restricted	5A	Expression is in the condensed mesenchyme.
TS19: metanephric mesenchyme	Present		5A

6A ©

Specimen 6A: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: early distal tubule	Present		6A
TS21: early proximal tubule	Present		6A
TS21: ureter	Present		6A
TS21: mesenchyme of metanephros	Present	Regionally restricted	6A	Expression is in the condensing mesenchyme.

7A ©

Specimen 7A: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: renal cortex	Strong	Regionally restricted	7A	Expression is in the podocyte layer surrounding the endothelial cells of the glomerulus and is absent in Bowman's capsule.

7G ©

Specimen 7G: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: urothelium of ureter	Present		7G

9F ©

Specimen 9F: embryonic day 13.5; Bmp7^tm1Rob/Bmp7^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS21: metanephros	Absent		9F

Specimen not shown E9.5: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: gut	Present
TS15: notochord	Present
TS15: surface ectoderm	Present

Specimen not showm E13.5: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: comma-shaped body	Present
TS21: s-shaped body	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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