Dkk1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:92327 Adamska M, et al., En1 and Wnt7a interact with Dkk1 during limb development in the mouse. Dev Biol. 2004 Aug 1;272(1):134-44
Assay type:	RNA in situ
MGI Accession ID:	MGI:6147811
Gene symbol:	Dkk1
Gene name:	dickkopf WNT signaling pathway inhibitor 1

Probe:	mdkk-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1B wildtype ©

Specimen 1B wildtype: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present	Widespread	1B wildtype	Expression throughout the apical ectodermal ridge.

1B En1-/- ©

Specimen 1B En1-/-: embryonic day 9.5; En1^tm1Alj/En1^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		1B En1-/-	Authors state expression did not differ between mutant and wild-type limbs at E9.5.
TS15: forelimb bud mesenchyme	Present	Scattered	1B En1-/-	Expression in anterior and posterior mesenchyme of limb.

1C wildtype ©

Specimen 1C wildtype: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		1C wildtype
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1C wildtype	Expression in anterior and posterior mesenchyme of limb.

1C En1-/- ©

Specimen 1C En1-/-: embryonic day 10.5; En1^tm1Alj/En1^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted	1C En1-/-	Authors report expression in expanded apical ectodermal ridge at a levels comparable to that of wild-type.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1C En1-/-	Expression in anterior and posterior mesenchyme of limb at comparable levels to that of wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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