Probe:	Hoxd13 probe25
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography
Assay notes:	Experiments were performed both on paraffin-embedded embryo section and frozen section. It was not specified which was used.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		2A right

Structure	Level	Pattern	Image	Note
TS13: embryo mesoderm	Present	Regionally restricted	2B right	Expressed in the most posterior.
TS13: primitive hindgut	Present		2B right

Structure	Level	Pattern	Image	Note
TS17: tail	Present		2C right

Structure	Level	Pattern	Image	Note
TS15: hindgut epithelium	Present	Regionally restricted	3A right	Expressed in the cloacal epithelium.
TS15: hindgut mesenchyme	Present	Regionally restricted	3A right	Expressed in the mesenchyme surrounding the cloacal epithelium, extending over a larger area compared with Evx2.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	3B right	Expressed in posterior.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Strong	Regionally restricted	3C right	Expressed in distal and posterior area.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Strong	Regionally restricted	3D right	Expressed in distal and posterior area.
TS20: genital tubercle	Strong		3D right

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	4A right	Expressed in slightly more extended domains than those of Evx2, more proximally along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Strong	Regionally restricted	4B right	Expressed in slightly more extended domains than those of Evx2, both more proximally and more anteriorly, along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Strong	Regionally restricted	4C right	Expressed in slightly more extended domains than those of Evx2, both more proximally and more anteriorly, along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS20: foot	Strong	Regionally restricted	4D right	Expressed along the posterior and the anterior margin.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 1	Strong		4E right
TS21: hindlimb digit 2	Strong		4E right
TS21: hindlimb digit 3	Strong		4E right
TS21: hindlimb digit 4	Strong		4E right
TS21: hindlimb digit 5	Strong		4E right

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present		5A right
TS19: mesenchyme of urogenital sinus	Present		5A right
TS19: hindgut mesenchyme	Present	Regionally restricted	5A right	Expressed around the urogenital sinus and rectum.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present		5B right

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present		5C right

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted		Expressed in posterior.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud mesenchyme	Present	Regionally restricted		Expressed in posterior.

Structure	Level	Pattern	Image	Note
TS25: hindlimb digit	Strong