Probe:	Y1 probe mix
Probe preparation:	Antisense labelled with S35 DNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present	Widespread	2_Y1 top row	Authors report abundant expression localized to most, or all, of mitral cells.

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present		2_Y1 2nd row

Structure	Level	Pattern	Image	Note
TS28: olfactory tubercle	Strong	Regionally restricted	2_Y1 3rd row	Prominent expression in grooves of olfactory tubercle.
TS28: islands of Calleja	Strong		2_Y1 3rd row
TS28: piriform cortex	Present		2_Y1 3rd row

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong	Widespread	2_Y1 4th row	Expression by most, or all, dentate granule cells.
TS28: cortical layer II	Present		2_Y1 4th row
TS28: cortical layer III	Present		2_Y1 4th row
TS28: cortical layer IV	Present		2_Y1 4th row
TS28: cortical layer VI	Present		2_Y1 4th row
TS28: CA1 pyramidal cell layer	Present		2_Y1 4th row
TS28: hippocampus CA3	Absent		2_Y1 4th row
TS28: basolateral amygdaloid nucleus	Trace	Diffuse	2_Y1 4th row	Authors report only after a very long exposure could a faint and diffuse signal be detected.
TS28: central amygdaloid nucleus	Trace	Diffuse	2_Y1 4th row	Authors report only after a very long exposure could a faint and diffuse signal be detected.
TS28: arcuate nucleus	Present		2_Y1 4th row

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Absent		2_Y1 bottom row

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		4A
TS28: cingulate cortex	Present		4A
TS28: cortical layer II	Present		4A
TS28: cortical layer III	Present		4A
TS28: cortical layer IV	Present		4A
TS28: cortical layer VI	Present		4A
TS28: dentate gyrus	Present		4A
TS28: hippocampus CA1	Present		4A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		4B

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present	Regionally restricted	4C	Expression in dorsomedial and ventrolateral arcuate nucleus. No expression in ventromedial region.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted		Prominent expression in cortex-amygdala transition zone.
TS28: olfactory tract	Present	Regionally restricted		Expression in lateral olfactory tract
TS28: presubiculum	Present
TS28: subiculum	Present
TS28: accumbens nucleus	Absent
TS28: anterior amygdaloid area	Absent
TS28: basomedial amygdaloid nucleus	Absent
TS28: corpus striatum	Absent
TS28: entorhinal cortex	Absent
TS28: globus pallidus	Absent
TS28: telencephalon septum	Absent
TS28: basolateral amygdaloid nucleus	Ambiguous
TS28: central amygdaloid nucleus	Ambiguous
TS28: hypothalamus	Present	Regionally restricted		No expression in lateral hypothalamus.
TS28: hypothalamus periventricular zone	Present
TS28: hypothalamus subparaventricular zone	Present
TS28: suprachiasmatic nucleus	Present
TS28: paraventricular hypothalamic nucleus	Ambiguous			Few expressing cells in paraventricular hypothalamus and medial parvicellular area.
TS28: medial preoptic area	Present	Regionally restricted		Expression in some cells of the medial preoptic area, including preoptic nuclei.
TS28: claustrum	Ambiguous
TS28: area postrema	Absent
TS28: dorsal motor nucleus of vagus nerve	Absent
TS28: dorsal raphe nucleus	Absent
TS28: superior cervical ganglion	Absent			Authors report no expression in adult sympathetic superior cervical ganglion.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Weak	Regionally restricted	5A	Much lower levels of expression than for Y2 (see assay MGI:6107953). Diffuse expression in a few of larger sized neurons.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Weak	Regionally restricted	5C	Much lower levels of expression than for Y2 (see assay MGI:6107953). Diffuse expression in a few of larger sized neurons.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	5E	More cells appear to be Y1-positive than Y2-positive (see assay MGI:6107953). Y1-expression mostly in the small and medium size population of neurons.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	5G	More cells appear to be Y1-positive than Y2-positive (see assay MGI:6107953). Y1-expression mostly in the small and medium size population of neurons.