Probe:	Id2 cDNA4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present		1_E6.5 lower left
TS9: embryo ectoderm	Absent		1_E6.5 lower left

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		2C/D

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Moderate		3I	More diffuse levels, with expression pattern similar to that of Id1.
TS19: neural tube ventricular layer	Moderate		3I	More diffuse levels, with expression pattern similar to that of Id1.
TS19: neural tube lateral wall	Present	Regionally restricted	3I	Expressed in small groups of cells in the intermediate zone of the basal plate.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		3J
TS19: midbrain	Present		3J
TS19: telencephalon	Present	Regionally restricted	3J	Expressed in the medial side of the telencephalic vesicles, located laterally to the third ventricle.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		3K

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Present		3L

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		4C
TS20: midbrain	Present		4C
TS20: telencephalon	Present	Regionally restricted	4C	Expressed in the entire telencephalic vesicles.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4H	Expressed in many postmitotic nuclei.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	4M	Expressed in many postmitotic nuclei.
TS22: hypothalamus mantle layer	Present		4M
TS22: hypothalamus subventricular zone	Present		4M
TS22: thalamus ventricular layer	Present		4M
TS22: cerebral cortex intermediate zone	Present		4M
TS22: cortical plate	Present		4M
TS22: cerebral cortex ventricular layer	Absent		4M
TS22: choroid plexus	Present		4M

Structure	Level	Pattern	Image	Note
TS24: thalamic nucleus	Present		4R
TS24: hypothalamus	Present	Regionally restricted	4R	Expressed in nuclei.
TS24: brainstem nucleus	Present	Regionally restricted	4R	Expressed in trochlear nucleus and pontine nucleus. Most intense signals.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	6A/B	Expressed in the intermediate layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		6C
TS28: cerebellum granule cell layer	Absent		6C
TS28: cerebellum molecular layer	Absent		6C
TS28: cerebellum ventricular layer	Absent		6C

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	6G	Expressed in the central region.
TS19: otocyst	Present		6G	Expressed in both the epithelium of the otic vesicle and its surrounding mesenchyme.
TS19: otocyst epithelium	Present		6G

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		6J

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		7C
TS22: lens	Present	Scattered	7C
TS22: pigmented retinal epithelium	Absent		7C

Structure	Level	Pattern	Image	Note
TS19: nasal pit epithelium	Present	Non-Uniform	7H
TS19: nasal process	Present	Regionally restricted	7H	Expressed in the area surrounding the nasal placode.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Weak	Regionally restricted	7M	Expressed in the presumptive olfactory neuronal progenitors located next to the mesenchyme.
TS22: nasal mesenchyme	Weak	Regionally restricted	7M	Expressed in the supporting cell layer, which is located adjacent to the lumen of the nasal cavity.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expressed in the fibroblastic stromal cells prior to their decidulation.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Weak

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present
TS15: optic vesicle	Present

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present
TS17: facial ganglion	Present
TS17: glossopharyngeal ganglion	Present
TS17: optic cup inner layer	Present
TS17: trigeminal ganglion	Present
TS17: vagus ganglion	Present
TS17: lens vesicle	Present	Scattered
TS17: optic cup outer layer	Absent
TS17: nasal process	Present	Regionally restricted		Expressed in the area surrounding the nasal placode.
TS17: olfactory placode	Present			Expression is not uniform throughout the placodal epithelium.

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present
TS19: infundibular recess of 3rd ventricle	Present

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: neural retinal epithelium	Present
TS20: vestibular ganglion	Present
TS20: cochlear ganglion	Absent
TS20: pigmented retinal epithelium	Absent
TS20: lens vesicle	Present	Scattered

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventral horn	Present	Regionally restricted		Expressed in the putative dorsal-most region of the motor neuron column.
TS22: spinal cord gray matter	Weak	Scattered		Expressed in the pars dorsalis and the pars ventralis (intermediate gray) but not in the future substantia gelatinosa (dorsal gray horn).

Structure	Level	Pattern	Image	Note
TS24: pigmented retinal epithelium	Absent
TS24: sympathetic ganglion	Absent
TS24: lens	Present	Scattered
TS24: neural retina	Present