Gene Expression Data
Assay Details
Assay
|
Reference:
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J:209070
Glaser A, et al., Tbx4 interacts with the short stature homeobox gene Shox2 in limb development. Dev Dyn. 2014 May;243(5):629-39
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5912539
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Gene symbol:
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Shox2
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Gene name:
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SHOX homeobox 2
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Probe:
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OG-12 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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|
Results
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Specimen
2Aa:
embryonic day 9.5
(more )
Specimen
2Aa:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud |
Present |
|
2Aa
|
Expression starts at the onset of limb outgrowth.
|
Specimen
2Ab:
embryonic day 10.5
(more )
Specimen
2Ab:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Widespread |
2Ab
|
Broadly expressed throughout the whole limb buds.
|
TS17: hindlimb bud |
Present |
Widespread |
2Ab
|
Broadly expressed throughout the whole limb buds.
|
Specimen
2Ac:
embryonic day 11.5
(more )
Specimen
2Ac:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2Ad:
embryonic day 12.5
(more )
Specimen
2Ad:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
Regionally restricted |
2Ad
|
Expressed at the proximal part.
|
TS20: hindlimb |
Present |
Regionally restricted |
2Ad
|
Expressed at the proximal part.
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Specimen
2Ae:
embryonic day 13.5
(more )
Specimen
2Ae:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Present |
Regionally restricted |
2Ae
|
Expressed at the proximal part.
|
TS21: hindlimb |
Present |
Regionally restricted |
2Ae
|
Expressed at the proximal part.
|
Specimen
2Ba:
embryonic day 10.5
(more )
Specimen
2Ba:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Widespread |
2Ba
|
Expression overlaps with the broader of Tbx4 expression domain.
|
Specimen
2Ba':
embryonic day 10.5
(more )
Specimen
2Ba':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Ba'
|
Expression broadly overlaps with Tbx4 throughout the proximal limb mesenchyme.
|
Specimen
2Bd:
embryonic day 11.5
(more )
Specimen
2Bd:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
2Bd
|
Expressed at the proximal part. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
|
Specimen
2Bd':
embryonic day 11.5
(more )
Specimen
2Bd':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Bd'
|
Expression broadly overlaps with Tbx4 throughout the proximal limb mesenchyme.
|
Specimen
2Bg:
embryonic day 12.5
(more )
Specimen
2Bg:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
Regionally restricted |
2Bg
|
Expressed at the proximal part. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
|
Specimen
2Bg':
embryonic day 12.5
(more )
Specimen
2Bg':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
2Bg'
|
Expressed in areas surrounding the proximal cartilaginous elements.
|
Specimen
4A WT:
embryonic day 10.5
(more )
Specimen
4A WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4A Tbx4-/-:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Tbx4tm1.1Pa/Tbx4tm1.1Pa
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|