Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47312
Hashimoto H, et al., Three-dimensional analysis of the developing pituitary gland in the mouse. Dev Dyn. 1998 May;212(1):157-66
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5911000
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Gene symbol:
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Lamb1
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Gene name:
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laminin B1
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Antibody:
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anti-laminin (AT-2404)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Fixative was 2% paraformaldehyde/1% glutaraldehyde. The authors state that the laminin antibody recognized both the a and b chains (Lama1 and Lamb1). Both genes have been annotated.
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Results
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Specimen
5:
embryonic day 12.5
(more  )
Specimen
5:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Regionally restricted |
5
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Expression is at the basement membrane. Expression is in the capillary network.
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| TS20: neurohypophysis infundibulum |
Present |
Regionally restricted |
5
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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| TS20: remnant of Rathke's pouch |
Present |
Regionally restricted |
5
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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Specimen
6:
embryonic day 13.5
(more )
Specimen
6:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pituitary gland |
Present |
Regionally restricted |
6
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Expression is at the basement membrane. Expression is in the capillary network.
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| TS21: neurohypophysis infundibulum |
Present |
Regionally restricted |
6
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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| TS21: remnant of Rathke's pouch |
Present |
Regionally restricted |
6
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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Specimen
18:
embryonic day 15.5
(more )
Specimen
18:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pituitary gland |
Present |
Regionally restricted |
18
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Expression is at the basement membrane of the epithelial tissue and in the intercellular spaces between the epithelial cells.
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Specimen
22:
embryonic day 18.5
(more )
Specimen
22:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pituitary gland |
Present |
Regionally restricted |
22
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Expression is at the basement membrane. Expression is in the capillary network.
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| TS26: neurohypophysis infundibulum |
Present |
Regionally restricted |
22
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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| TS26: remnant of Rathke's pouch |
Present |
Regionally restricted |
22
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Expression is at the basement membrane. Expression is absent at points of direct contact between the infundibular process and Rathke's pouch. Expression is in the capillary network.
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