Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18543
Rashbass P, et al., Alterations in gene expression during mesoderm formation and axial patterning in Brachyury (T) embryos. Int J Dev Biol. 1994 Mar;38(1):35-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5910889
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 probe49
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Probe preparation:
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Antisense RNA
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Assay notes:
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Control embryos were either heterozygotes or +/+. Embryos were post-fixed with 4% paraformaldhyde/0.2% glutaraldehyde.
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Results
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Specimen
3H control:
embryonic day 9.5
(more  )
Specimen
3H control:
(close  )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: head |
Present |
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3H control
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| TS15: neural tube |
Present |
Regionally restricted |
3H control
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Expression is dorsoventral.
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Specimen
3H -/-:
embryonic day 9.5; T/ T
(more )
Specimen
3H -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: head |
Present |
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3H -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS15: neural tube |
Present |
Regionally restricted |
3H -/-
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The expression domain is enlarged in this mutant.
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Specimen
3I:
embryonic day 9.5
(more )
Specimen
3I:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3J:
embryonic day 9.5; T/ T
(more )
Specimen
3J:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural fold |
Present |
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3J
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In this mutant expression is in an enlarged and ventralized band in the rostral neural folds and greatly increased ventral expression in the trunk and caudal neural folds.
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| TS15: neural tube |
Present |
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3J
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In this mutant expression is in an enlarged and ventralized band in the rostral neural folds and greatly increased ventral expression in the trunk and caudal neural folds.
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| TS15: neural tube floor plate |
Present |
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3J
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Expression is in the caudal neural tube.n the caudal neural tube.
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Specimen
3K,L:
embryonic day 9.5; T/ T
(more )
Specimen
3K,L:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown: control:
embryonic day 8.5
(more )
Specimen
not shown: control:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
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Expression is in the ventrolateral regions.
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Specimen
not shown: -/-:
embryonic day 8.5; T/ T
(more )
Specimen
not shown: -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type.
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