About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:240500 Lopez-Domenech G, et al., Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites. Cell Rep. 2016 Oct 04;17(2):317-327
Assay type: Western blot
MGI Accession ID: MGI:5906591
Gene symbol: Rhot2
Gene name: ras homolog family member T2
Antibody: anti-Miro1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Antibody recognizes both Miro1 (Rhot1; duplex at 71-73 kDa) and Miro2 (Rhot2; 74 kDa). Both genes have been annotated.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 73 kDa Genetic BackgroundMutant Allele(s)Sex Note
WT 1 month P m 1 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 4 months P m 4 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 8 months P m 8 TS28: hippocampus Weak (a) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 12 months P m 12 TS28: hippocampus Weak (a) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
Miro1cko 1 month P m 1 TS28: hippocampus Strong (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 4 months P m 4 TS28: hippocampus Strong (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 8 months P m 8 TS28: hippocampus Strong (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 12 months P m 12 TS28: hippocampus Strong (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Notes:
(a) Authors report that expression levels decreased progressively over time in wild type animals.
(b) Expression levels increase after Miro1 (Rhot1) deletion.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory