Probe:	Fgf15 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural plate	Present	Regionally restricted	1A	Expression in anterior neural ridge neuroepithelium.
TS13: rhombomere 4	Present	Regionally restricted	1A	Expression in basal plate epithelium of rhombomere 4.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present		1A inset
TS13: otic placode	Present	Regionally restricted	1A inset	Expression in dorsal part of otic placode.

Structure	Level	Pattern	Image	Note
TS13: neural plate	Present	Regionally restricted	1B	Expression in anterior neural ridge neuroepithelium.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	1C	Expression in prosencephalic commissural plate.
TS15: optic vesicle	Present	Regionally restricted	1C	Expression in neural epithelium of optic vesicle.
TS15: diencephalon	Present	Regionally restricted	1C	Expression restricted to dorsal-most region of alar thalamic epithelium.
TS15: future midbrain	Present		1C	Authors report mesencephalic expression.
TS15: midbrain-hindbrain junction	Absent		1C	Authors describe a negative gap of expression in isthmus.
TS15: future hindbrain	Present	Regionally restricted	1C	Authors report rostral rhombencephalic expression.
TS15: rhombomere 1	Present	Regionally restricted	1C	Expression in cerebellar plate.
TS15: rhombomere 4	Present	Regionally restricted	1C	Expression in alar plate of rhombomere 4.

Structure	Level	Pattern	Image	Note
TS15: otic pit	Present	Regionally restricted	1C inset	Expression in dorsal part of otic placode.
TS15: optic vesicle	Present	Regionally restricted	1C inset	Expression in neural epithelium of optic vesicle.
TS15: epibranchial placode	Present		1C inset

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	1D	Expression in commissural plate.
TS17: diencephalon	Present	Regionally restricted	1D	Expression restricted to dorsal-most region of alar thalamic epithelium.
TS17: midbrain	Present		1D	Authors report mesencephalic expression.
TS17: midbrain-hindbrain junction	Absent		1D	Authors describe a negative gap of expression in isthmus.
TS17: hindbrain	Present	Regionally restricted	1D	Authors report rostral rhombencephalic expression.
TS17: rhombomere 1	Present	Regionally restricted	1D	Expression in cerebellar plate.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	1D inset	Expression in dorsal part of otic placode.
TS17: epibranchial placode	Present		1D inset

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1E	Expression in commissural plate.
TS19: neural retina	Present	Regionally restricted	1E	Expression in neural epithelium of optic vesicle.
TS19: diencephalon	Present	Regionally restricted	1E	Expression expanded caudally into dorsal pretectal area.
TS19: midbrain	Present		1E	Authors report mesencephalic expression.
TS19: midbrain-hindbrain junction	Absent		1E	Authors describe a negative gap of expression in isthmus.
TS19: hindbrain	Present	Regionally restricted	1E	Authors report rostral rhombencephalic expression. Expression on both sides of isthmic organizer.
TS19: metencephalon alar plate	Present	Regionally restricted	1E	Expression in cerebellar plate.

Structure	Level	Pattern	Image	Note
TS19: otocyst	Present	Regionally restricted	1E inset	Expression in dorsal part of otic placode.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	1G	Decreasing rostrocaudal pattern of expression in mesencephalic alar plate.
TS19: midbrain-hindbrain junction	Absent		1G	Authors describe a negative gap of expression in isthmus.
TS19: metencephalon alar plate	Present	Regionally restricted	1G	Expression in cerebellar plate.
TS19: hindbrain	Present	Regionally restricted	1G	Authors report rostral rhombencephalic expression.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2A	Expression in commissural plate.
TS20: diencephalon	Present	Regionally restricted	2A	Expression in several domains of diencephalic neuroepithelium.
TS20: thalamus	Present	Regionally restricted	2A	Expression in two regions of thalamus, stronger at both sides of the zona limitans intrathalamica.
TS20: zona limitans intrathalamica	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2B	Expression localized to commissural plate, septum and preoptic area.
TS21: preoptic area	Present		2B
TS21: optic stalk	Present		2B	Expression continuous in optic stalk, suggesting extension of expression into optic cup
TS21: prethalamus	Present	Regionally restricted	2B	Authors state diencephalic expression pattern was similar to observed at previous stages
TS21: thalamus	Present	Regionally restricted	2B	Authors state diencephalic expression pattern was similar to observed at previous stages
TS21: zona limitans intrathalamica	Absent		2B
TS21: midbrain	Present	Regionally restricted	2B	Expression in all dorsal mesencephalic epithelium.
TS21: midbrain-hindbrain junction	Absent		2B	Gap in expression at level of isthmic organizer.
TS21: hindbrain	Present	Regionally restricted	2B	Authors state rhombencephalic expression pattern was similar to observed at previous stages.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	2C	xpression in lateral septum.
TS22: preoptic area	Present		2C
TS22: midbrain roof plate	Present	Widespread	2C	Expression extended along entire mesencephalic roof plate to limit with isthmic organizer.
TS22: midbrain-hindbrain junction	Absent		2C	Negative gap of expresion at isthmus still appears.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	2D	Expression in lateral septum.
TS22: preoptic area	Present		2D
TS22: midbrain roof plate	Present	Widespread	2D	Expression extended along entire mesencephalic roof plate to limit with isthmic organizer.
TS22: midbrain-hindbrain junction	Absent		2D	Negative gap of expresion at isthmus still appears.

Structure	Level	Pattern	Image	Note
TS23: telencephalon septum	Present	Regionally restricted	2E	Expression in lateral septum.
TS23: main olfactory bulb	Present		2E
TS23: preoptic area	Present		2E
TS23: dorsal thalamus	Present		2E	Stronger diencephalic expression than at previous stages.
TS23: epithalamus	Present		2E	Stronger diencephalic expression than at previous stages.
TS23: pretectum	Present		2E	Stronger diencephalic expression than at previous stages.
TS23: midbrain roof plate	Present	Widespread	2E	Expression extended along entire mesencephalic roof plate to limit with isthmic organizer.
TS23: midbrain-hindbrain junction	Absent		2E	Negative gap of expression at isthmus still appears.

Structure	Level	Pattern	Image	Note
TS23: telencephalon septum	Present	Regionally restricted	2F	Expression in lateral septum followed germinal epithelium into olfactory bulb.
TS23: main olfactory bulb	Present		2F
TS23: midbrain roof plate	Present	Widespread	2F	Expression extended along entire mesencephalic root plate to limit with isthmic organizer.
TS23: midbrain-hindbrain junction	Absent		2F	Negative gap of expression at isthmus still appears.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	2G	Anterior expression domain extends from septum towards olfactory bulb neuroepithelium.
TS22: main olfactory bulb	Present		2G
TS22: telencephalon	Present	Regionally restricted	2G	Transient expression in a small caudal region of lateral ventricle epithelium corresponding to territory of amydaline complex.
TS22: cerebellum mantle layer	Present	Regionally restricted	2G
TS22: cerebellum	Present	Regionally restricted	2G	Weak expression at midline. Two lateral strips of expression in cerebellar hemispheres. Strong expression in intermedium and lateral cerebellum. Two intermedium bands of expression in hemispheres. Expression limited by midline and lateral negative regions.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	2H	Expression in lateral septum.
TS22: dorsal thalamus	Present	Regionally restricted	2H	Expression in ventricular epithelium of ventral thalamus and dorsal thalamus, from stria medullaris to dorsal pretectum. No expression in basal plate epithelium.
TS22: prethalamus	Present	Regionally restricted	2H	Expression in ventricular epithelium of ventral thalamus and dorsal thalamus, from stria medullaris to dorsal pretectum. No expression in basal plate epithelium.

Structure	Level	Pattern	Image	Note
TS22: dorsal thalamus	Present		2I
TS22: epithalamus	Present		2I

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present		3A
TS24: epithalamus	Present		3A
TS24: midbrain roof plate	Present	Regionally restricted	3A	Epithelial expression in pretectal and mesencephalic alar plate restricted to the most dorsomedial regions.
TS24: pretectum	Present	Regionally restricted	3A	Epithelial expression in pretectal and mesencephalic alar plate restricted to the most dorsomedial regions.
TS24: cerebellar cortex	Present		3A

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Present		3B
TS24: main olfactory bulb	Present	Regionally restricted	3B	Stronger expression in dorsal than in ventral part of olfactory bulb neuroepithelium.
TS24: pretectum	Present	Regionally restricted	3B	Expression in some scattered cells in ventral region of pretectal alar plate, where interstitial nucleus of Cajal is localized.
TS24: cerebellar cortex	Present		3B

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Present		3C
TS24: main olfactory bulb	Present	Regionally restricted	3C	Stronger expression in dorsal than in ventral part of olfactory bulb neuroepithelium.
TS24: cerebellar cortex	Present		3C

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	3D	Stronger expression in dorsal than in ventral part of olfactory bulb neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Present		3E

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present	Regionally restricted	3F
TS24: epithalamus	Present	Regionally restricted	3F
TS24: dorsal lateral geniculate nucleus	Present		3F
TS24: medial habenular nucleus	Present		3F

Structure	Level	Pattern	Image	Note
TS24: midbrain roof plate	Present	Regionally restricted	3G
TS24: cerebellar cortex	Strong	Regionally restricted	3G	Strong expression in cortex of lateral cerebellum.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	3H
TS26: telencephalon septum	Present	Regionally restricted	3H	Expression in lateral septum.
TS26: midbrain roof plate	Present	Regionally restricted	3H
TS26: cerebellum	Present		3H

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	3I
TS26: midbrain roof plate	Present	Regionally restricted	3I
TS26: telencephalon septum	Present	Regionally restricted	3I
TS26: cerebellum	Present		3I

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	3J
TS26: telencephalon septum	Present	Regionally restricted	3J

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Regionally restricted	3K	Expression in lateral septum.

Structure	Level	Pattern	Image	Note
TS26: dorsal lateral geniculate nucleus	Present		3L
TS26: medial habenular nucleus	Present		3L

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present		3M
TS26: superior colliculus	Present		3M

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		3N

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		3O

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	4A
TS27: midbrain roof plate	Present	Regionally restricted	4A
TS27: telencephalon septum	Present	Regionally restricted	4A
TS27: medial habenular nucleus	Present		4A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	4B
TS27: midbrain roof plate	Present	Regionally restricted	4B
TS27: telencephalon septum	Present	Regionally restricted	4B

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		4C

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	4D	Longitudinal stripes of expression in cerebellar Purkinje cells.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	4E	Longitudinal stripes of expression in cerebellar Purkinje cells.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	4F

Structure	Level	Pattern	Image	Note
TS27: rostral migratory stream	Present	Regionally restricted	4G

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present		4H

Structure	Level	Pattern	Image	Note
TS27: medial habenular nucleus	Present		4I
TS27: dorsal lateral geniculate nucleus	Present		4I

Structure	Level	Pattern	Image	Note
TS27: dorsal lateral geniculate nucleus	Present		4J

Structure	Level	Pattern	Image	Note
TS27: dorsal lateral geniculate nucleus	Present		4K

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	4L
TS27: midbrain roof plate	Present	Regionally restricted	4L

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	4M
TS27: midbrain roof plate	Present	Regionally restricted	4M

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	4N	Longitudinal stripes of expression in cerebellar Purkinje cells.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	4O	Longitudinal stripes of expression in cerebellar Purkinje cells.

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present	Regionally restricted	5A
TS27: main olfactory bulb	Present	Regionally restricted	5A
TS27: midbrain roof plate	Present	Regionally restricted	5A
TS27: telencephalon septum	Present	Regionally restricted	5A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	5B

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present	Regionally restricted	5C
TS27: main olfactory bulb	Present	Regionally restricted	5C
TS27: midbrain roof plate	Present	Regionally restricted	5C
TS27: telencephalon septum	Present	Regionally restricted	5C

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	5D

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present		5E

Structure	Level	Pattern	Image	Note
TS27: dorsal lateral geniculate nucleus	Present		5F
TS27: medial habenular nucleus	Present		5F

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		5G
TS27: telencephalon septum	Present		5G

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present		5H

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present		5I

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present		5J
TS28: main olfactory bulb	Present		5J
TS28: midbrain roof plate	Present		5J
TS28: telencephalon septum	Present		5J

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Present		5K

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Present		5L

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Present		5M