Probe:	NZF-3 (St18/Myt3) cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	3C	Expression is in the caudo-ventral region.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	3G	Expression is in the dorsal telencephalon and in basal ganglia.
TS17: forebrain	Present	Regionally restricted	3G	Expression is in the subventricular zone.
TS17: optic cup	Present	Regionally restricted	3G	Expression is in differentiated neurons.
TS17: diencephalon	Present	Regionally restricted	3G	Expression is in the hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3K	Expression is in the cerebral cortex and basal ganglia.
TS19: trigeminal ganglion	Present		3K

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3O	Expression is in the archipallium and basal ganglia.
TS20: cerebral cortex	Present		3O
TS20: hypothalamus	Present		3O
TS20: thalamus	Present		3O

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3S	Expression is in the archipallium and basal ganglia.
TS21: cerebral cortex	Present		3S
TS21: hypothalamus	Present		3S
TS21: thalamus	Present		3S

Structure	Level	Pattern	Image	Note
TS22: basal ganglia	Present		3W
TS22: hippocampus	Present		3W

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present		3Z
TS23: hippocampus	Present		3Z

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		4C

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		4H	Expression is strongest in the upper cortical plate and lower near the boundary adjacent to the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Weak	Regionally restricted	4M	Expression is in the dorsal and lateral part.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present		5C
TS20: telencephalon subventricular zone	Present		5C

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present		5G
TS20: telencephalon subventricular zone	Present		5G
TS20: telencephalon ventricular layer	Present	Single cells	5G

Structure	Level	Pattern	Image	Note
TS26: hippocampus region	Present	Regionally restricted	5K	Expression is in the pyramidal cells of the cornu ammonis.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain lateral wall	Present	Regionally restricted	6C	Expression is in the ventral basal plate just adjacent to the floor plate.
TS15: facio-acoustic preganglion complex	Strong		6C
TS15: trigeminal preganglion	Strong		6C

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	6G	Expression is in the pons and medulla.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	6K	There is restricted expression in the subventricular zone.
TS20: midbrain	Present	Regionally restricted	6K	There is restricted expression in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	6O	There is restricted expression in the subventricular zone.
TS21: midbrain	Present	Regionally restricted	6O	There is restricted expression in the subventricular zone.
TS21: cerebellum layer	Present	Regionally restricted	6O	Expression is in the external granule layer.

Structure	Level	Pattern	Image	Note
TS26: cerebellum granule cell layer	Present		6S

Structure	Level	Pattern	Image	Note
TS20: eye	Weak		7C

Structure	Level	Pattern	Image	Note
TS21: neural retina	Weak		7G

Structure	Level	Pattern	Image	Note
TS23: retina layer	Moderate	Regionally restricted	7K	Expression is in some outer neuroblastic layer cells.
TS23: retina	Weak	Single cells	7K

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Moderate	Regionally restricted	7N	Expression is in the basal layer of the epithelium.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	8C	Expression is in the earliest born neurons.
TS15: dorsal root ganglion	Weak		8C

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	8K	Expression is in the subventricular zone.
TS20: dorsal root ganglion	Present		8K

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	8O	Expression is in the subventricular zone.
TS21: dorsal root ganglion	Weak		8O
TS21: spinal cord mantle layer	Weak		8O
TS21: alar columns	Strong	Regionally restricted	8O	Expression is in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Weak	Regionally restricted	8S
TS21: trigeminal ganglion	Weak	Regionally restricted	8S

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	9C	Expression is in subventricular cells.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Weak	Regionally restricted		Expression is in scattered cells in the basal half.
TS20: cranial ganglion	Present
TS20: vagus ganglion	Strong
TS20: vestibulo-cochlear ganglion complex	Strong	Regionally restricted