Probe:	NZF-1 (Myt1l/png-1) cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Absent		3A

Structure	Level	Pattern	Image	Note
TS17: forebrain	Absent		3E

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3I	Expression is in the cerebral cortex and basal ganglia.
TS19: trigeminal ganglion	Present		3I

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3M	Expression is in the archipallium and basal ganglia.
TS20: cerebral cortex	Present		3M
TS20: hypothalamus	Present		3M
TS20: thalamus	Present		3M

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3Q	Expression is in the archipallium and basal ganglia.
TS21: cerebral cortex	Present		3Q
TS21: hypothalamus	Present		3Q
TS21: thalamus	Present		3Q

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present		3U

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present		3X

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		4A

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		4F	Expression is stronger at the upper limit.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		4K	Expression is in post-mitotic neurons.
TS22: cerebral cortex subventricular zone	Present		4K	Expression is in post-mitotic neurons.
TS22: cerebral cortex ventricular layer	Present		4K	Expression is in post-mitotic neurons.
TS22: cortical plate	Present		4K	Expression is in post-mitotic neurons.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Very strong	Regionally restricted	5A	Expression is in post-mitotic neurons in the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Very strong	Regionally restricted	5E	Expression is in post-mitotic neurons in the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS26: hippocampus region	Present	Regionally restricted	5I	Expression is in the pyramidal cells of the cornu ammonis.
TS26: dentate gyrus	Present	Regionally restricted	5I	Expression is in granule cells.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Absent		6A
TS15: future midbrain	Absent		6A

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Absent		6E
TS17: midbrain	Absent		6E

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		6I
TS20: medulla oblongata	Present		6I
TS20: midbrain-hindbrain junction	Present		6I
TS20: pons	Present		6I
TS20: midbrain roof plate	Present	Regionally restricted	6I	Expression is in the inferior and superior colliculi.
TS20: midbrain	Present	Regionally restricted	6I	Expression is in the tegmentum.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		6M
TS21: medulla oblongata	Present		6M
TS21: midbrain tegmentum	Present		6M
TS21: midbrain-hindbrain junction	Present		6M
TS21: pons	Present		6M
TS21: midbrain roof plate	Present	Regionally restricted	6M	Expression is in the inferior and superior colliculi.

Structure	Level	Pattern	Image	Note
TS26: cerebellum granule cell layer	Absent		6Q

Structure	Level	Pattern	Image	Note
TS20: eye	Present		7A

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present	Regionally restricted	7E	Expression is in the inner part.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Present	Regionally restricted	7I	Expression is in the innermost part of the inner neuroblastic layer of the neural retina.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Strong	Regionally restricted	7L	Expression is in the middle layer of the epithelium but not in the luminal and basal layer.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	8A	Expression is at the most ventrolateral margin of the spinal cord.
TS15: dorsal root ganglion	Weak		8A

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	8I	Expression is in post-mitotic neurons with an increasing ventrolateral gradient.
TS20: dorsal root ganglion	Present		8I

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	8M	Expression is in post-mitotic neurons.
TS21: dorsal root ganglion	Present		8M
TS21: spinal cord white matter	Absent		8M

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Moderate		8Q

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	9A	Expression is in post-mitotic neurons.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present
TS20: olfactory epithelium	Present